LIPH Monoclonal antibody, PBS Only

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  WB analysis of SW 1990 using 66303-1-Ig (same clone as 66303-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 66303-1-Ig (same clone as 66303-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66303-1-Ig (same clone as 66303-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66303-1-Ig (same clone as 66303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66303-1-Ig (same clone as 66303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66303-1-Ig (same clone as 66303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66303-1-Ig (same clone as 66303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 66303-1-Ig (same clone as 66303-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 66303-1-Ig(LIPH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

LIPH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66303-1-PBS

Clone No.1F5D10

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AH, Lipase member H, lipase, member H, LIPH, LPD lipase related protein, LPDLR, mPA PLA1, mPA PLA1 alpha, MPAPLA1, Phospholipase A1 member B, PLA1B

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Product Information

66303-1-PBS targets LIPH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24009

  Product name: Recombinant human LIPH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 103-451 aa of BC064941

  Sequence: VVVVDWNRGATTLIYTHASSKTRKVAMVLKEFIDQMLAEGASLDDIYMIGVSLGAHISGFVGEMYDGWLGRITGLDPAGPLFNGKPHQDRLDPSDAQFVDVIHSDTDALGYKEPLGNIDFYPNGGLDQPGCPKTILGGFQYFKCDHQRSVYLYLSSLRESCTITAYPCDSYQDYRNGKCVSCGTSQKESCPLLGYYADNWKDHLRGKDPPMTKAFFDTAEESPFCMYHYFVDIITWNKNVRRGDITIKLRDKAGNTTESKINHEPTTFQKYHQVSLLARFNQDLDKVAAISLMFSTGSLIGPRYKLRILRMKLRSLAHPERPQLCRYDLVLMENVETVFQPILCPELQL

• Full Name: lipase, member H
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC064941
• Gene Symbol: LIPH
• Gene ID (NCBI): 200879
• RRID: AB_2881686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WWY8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).