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  • KD/KO Validated

LMOD3 Polyclonal antibody

LMOD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 14948-1-AP


Leiomodin 3, leiomodin 3 (fetal), Leiomodin, fetal form, LMOD3

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14948-1-AP targets LMOD3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish, Xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMOD3 fusion protein Ag6758
Full Name leiomodin 3 (fetal)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC039202
Gene Symbol LMOD3
Gene ID (NCBI) 56203
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene encoding LMOD3 has not been characterized so far and very limited information of its function has been reported. Nanda et al. found that the expression of mouse LMOD3 mRNA is restricted largely to cardiac and skeletal muscle through RT-PCR analysis (PMID: 22157009). Two isoforms of LMOD3 may exist due to the alternative splicing, whose molecular weights are predicted as 65 kDa and 40 kDa, respectively (Uniprot). This antibody was raised against the N-terminal region of human LMOD3. It detects a double bands around 80 kDa and 65 kDa in heart and skeletal muscle lysates. The reason causing the discrepancy between the predicted and observed molecular weight is not clear.


Product Specific Protocols
WB protocol for LMOD3 antibody 14948-1-APDownload protocol
IHC protocol for LMOD3 antibody 14948-1-APDownload protocol
IP protocol for LMOD3 antibody 14948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

Nebulin and Lmod2 are critical for specifying thin-filament length in skeletal muscle.

Authors - Balázs Kiss
human, zebrafishWB, IF

J Clin Invest

Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy.

Authors - Michaela Yuen

J Clin Invest

Severe myopathy in mice lacking the MEF2/SRF-dependent gene leiomodin-3.

Authors - Bercin K Cenik
  • KO Validated
zebrafishWB, IF

J Clin Invest

Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy.

Authors - Michaela Yuen

J Clin Invest

KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy.

Authors - Ankit Garg

Proc Natl Acad Sci U S A

HSPB7 is indispensable for heart development by modulating actin filament assembly.

Authors - Tongbin Wu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tongbin (Verified Customer) (08-25-2020)

This antibody is very specific. I had a single Lmod3 band at around 70kD. Western blot results of this antibody had been published in our PNAS paper (PMID: 29078393)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart
LMOD3 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:14948-1-AP)