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LMOD3 Polyclonal antibody

LMOD3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14948-1-AP

Synonyms

Leiomodin 3, leiomodin 3 (fetal), Leiomodin, fetal form, LMOD3



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14948-1-AP targets LMOD3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, Xenopus, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMOD3 fusion protein Ag6758
Full Name leiomodin 3 (fetal)
Calculated molecular weight 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC039202
Gene symbol LMOD3
Gene ID (NCBI) 56203
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The gene encoding LMOD3 has not been characterized so far and very limited information of its function has been reported. Nanda et al. found that the expression of mouse LMOD3 mRNA is restricted largely to cardiac and skeletal muscle through RT-PCR analysis (PMID: 22157009). Two isoforms of LMOD3 may exist due to the alternative splicing, whose molecular weights are predicted as 65 kDa and 40 kDa, respectively (Uniprot). This antibody was raised against the N-terminal region of human LMOD3. It detects a double bands around 80 kDa and 65 kDa in heart and skeletal muscle lysates. The reason causing the discrepancy between the predicted and observed molecular weight is not clear.

Protocols

Product Specific Protocols
WB protocol for LMOD3 antibody 14948-1-APDownload protocol
IHC protocol for LMOD3 antibody 14948-1-APDownload protocol
IP protocol for LMOD3 antibody 14948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Sleep Res

Kleine-Levin syndrome is associated with LMOD3 variants.

Authors - Saad M Al Shareef
XenopusIHC

Methods

Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging.

Authors - Nworu Chinedu U CU
XenopusWB, IF

J Cell Sci

Leiomodin 3 and Tropomodulin 4 have overlapping functions during skeletal myofibrillogenesis.

Authors - Chinedu U Nworu
mouseWB,IHC

Development

Tropomodulin1 directly controls thin filament length in both wild-type and tropomodulin4-deficient skeletal muscle.

Authors - David S Gokhin
mouseIF

J Neurosci

Gene Expression by Mouse Inner Ear Hair Cells during Development.

Authors - Déborah I Scheffer
mouseWB

Elife

KLHL41 stabilizes skeletal muscle sarcomeres by nonproteolytic ubiquitination.

Authors - Andres Ramirez-Martinez

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

This antibody is very specific. I had a single Lmod3 band at around 70kD. Western blot results of this antibody had been published in our PNAS paper (PMID: 29078393)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart
LMOD3 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:14948-1-AP)