|Positive WB detected in||mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human heart tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14948-1-AP targets LMOD3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Xenopus, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LMOD3 fusion protein Ag6758|
|Full Name||leiomodin 3 (fetal)|
|Calculated molecular weight||65 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC039202|
|Gene ID (NCBI)||56203|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The gene encoding LMOD3 has not been characterized so far and very limited information of its function has been reported. Nanda et al. found that the expression of mouse LMOD3 mRNA is restricted largely to cardiac and skeletal muscle through RT-PCR analysis (PMID: 22157009). Two isoforms of LMOD3 may exist due to the alternative splicing, whose molecular weights are predicted as 65 kDa and 40 kDa, respectively (Uniprot). This antibody was raised against the N-terminal region of human LMOD3. It detects a double bands around 80 kDa and 65 kDa in heart and skeletal muscle lysates. The reason causing the discrepancy between the predicted and observed molecular weight is not clear.
J Sleep Res
Kleine-Levin syndrome is associated with LMOD3 variants.
Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging.
J Cell Sci
Leiomodin 3 and Tropomodulin 4 have overlapping functions during skeletal myofibrillogenesis.
Tropomodulin1 directly controls thin filament length in both wild-type and tropomodulin4-deficient skeletal muscle.
Gene Expression by Mouse Inner Ear Hair Cells during Development.
KLHL41 stabilizes skeletal muscle sarcomeres by nonproteolytic ubiquitination.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-25-2020)
This antibody is very specific. I had a single Lmod3 band at around 70kD. Western blot results of this antibody had been published in our PNAS paper (PMID: 29078393)