LC3 Polyclonal antibody
LC3 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
246
Cat no : 14600-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Chloroquine treated HepG2 cells, MCF-7 cells, HSC-T6 cells, mouse brain tissue, mouse lung tissue, NIH/3T3 cells, mouse heart tissue, mouse liver tissue, Chloroquine treated HEK-293 cells, HeLa cells, mouse testis tissue, human skeletal muscle tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue, human testis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Chloroquine treated HepG2 cells, Starvation treated HEK-293 cells, HepG2 cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:600-1:2500 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:100-1:400 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 212 publications below |
| IHC | See 30 publications below |
| IF | See 60 publications below |
Product Information
14600-1-AP targets LC3 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | bovine, duck, goat, human, monkey, mouse, pig, rabbit, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LC3 fusion protein Ag6144 |
| Full Name | microtubule-associated protein 1 light chain 3 beta |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 14-18 kDa |
| GenBank accession number | BC067797 |
| Gene symbol | MAP1LC3B |
| Gene ID (NCBI) | 81631 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LC3 antibody 14600-1-AP | Download protocol |
| IHC protocol for LC3 antibody 14600-1-AP | Download protocol |
| IF protocol for LC3 antibody 14600-1-AP | Download protocol |
| IP protocol for LC3 antibody 14600-1-AP | Download protocol |
| FC protocol for LC3 antibody 14600-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nanotoxicology Silver nanoparticles induce protective autophagy via Ca2+/CaMKKβ/AMPK/mTOR pathway in SH-SY5Y cells and rat brains. | ||
J Cancer Lyn Kinase Promotes the Proliferation of Malignant Melanoma Cells through Inhibition of Apoptosis and Autophagy via the PI3K/Akt Signaling Pathway. | ||
Int Immunopharmacol Protective effects of levo-tetrahydropalmatine on hepatic ischemia/reperfusion injury are mediated by inhibition of the ERK/NF-κB pathway. | ||
Connect Tissue Res Rapamycin attenuates articular cartilage degeneration by inhibiting β-catenin in a murine model of osteoarthritis. | ||
FASEB J STAT3 phosphorylation mediates high glucose-impaired cell autophagy in an HDAC1-dependent and -independent manner in Schwann cells of diabetic peripheral neuropathy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Eric (Verified Customer) (03-13-2021) | Worked very well. I was able to get the LC3A/B bands on western blot using a 12.5% gel and prominent bands on a 8% gel. IF imaging worked fairly well, I used 1:100 which was likely too low so I will repeat at higher but got signal nonetheless. This performed better than some of the other LC3 antibodies we tested.
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FH Elzbieta (Verified Customer) (09-23-2020) | The antibody works very nicely in WB. Clear signal, no aspecific bands. Our lab discarded other products and switched to this antibody.
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FH An (Verified Customer) (09-17-2020) | Used for IHC on zebrafish cryosections of eyes. Worked well.
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FH David (Verified Customer) (03-30-2020) | Very nice visualisation of LC3-I and LC3-II. Run on 20% gel and significant changes in response to treatment with bafilomycin A and concanamycin A.
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