Chloroquine treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP( LC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Chloroquine treated HepG2 cells (left) and untreated HepG2 cells (right) were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP(LC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HSC-T6 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP(LC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP(LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP(LC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP(LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP(LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP(LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse lung tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP(LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse testis tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP(LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP(LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

rat brain tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP( LC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14600-1-AP( LC3 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14600-1-AP( LC3 Antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:600 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:600 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human testis using 14600-1-AP(LC3 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human testis using 14600-1-AP(LC3 antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-LC3 (IP:14600-1-AP, 3ug; Detection:14600-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Immunofluorescent analysis of ( -20°C Ethanol) fixed Chloroquine treated HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( -20°C Ethanol) fixed Chloroquine treated HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( -20°C Ethanol) fixed Chloroquine treated HepG2 cells (left) and untreated HepG2 cells (right) using 14600-1-AP (LC3 antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 66187-1-Ig (KRT18 antibody) was used to label cytoskeleton (red).

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of Starvation treated HEK-293 cells using 14600-1-AP (LC3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 20 mM chloroquine was used to block the autophagy flux.

1X10^6 HeLa cells were stained with 0.2ug LC3 antibody (14600-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.