LC3 Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 14600-1-AP

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Con: 41 μg/150 μl

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Species specificity:
human, mouse, rat, monkey

Positive WB detected in:
Chloroquine treated HEK-293 cells, mouse testis tissue, human skeletal muscle tissue, mouse brain tissue, mouse lung tissue, NIH/3T3 cells, mouse heart tissue, mouse liver tissue, MCF-7 cells, HeLa cells, HEK-293 cells, HSC-T6 cells, rat brain tissue

Positive IP detected in:
mouse brain tissue

Positive IHC detected in:
human gliomas tissue, human breast cancer tissue, human testis tissue

Positive IF detected in:
Starvation treated HEK-293 cells, Chloroquine treated HepG2 cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Purification method:
Antigen affinity purification


PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Full name:
microtubule-associated protein 1 light chain 3 beta

Calculated molecular weight:
15 kDa

Observed molecular weight:
16-18 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol

LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B

Map1LC3, also known as LC3, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18-kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 16-kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

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