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  • Featured Product
  • KD/KO Validated

LC3 Polyclonal antibody

LC3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14600-1-AP

Synonyms

LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 14600-1-AP

    WB analysis of HeLa using 14600-1-AP

    WB result of LC3 antibody (14600-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-LC3 transfected HeLa cells.

  • WB analysis using 14600-1-AP

    WB analysis using 14600-1-AP

    Chloroquine treated HepG2 cells (left) and untreated HepG2 cells (right) after serum deprived for 2 hours were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 14600-1-AP

    WB analysis of HEK-293 using 14600-1-AP

    CQ treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14600-1-AP

    WB analysis of HeLa using 14600-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 14600-1-AP

    WB analysis of mouse testis using 14600-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human skeletal muscle using 14600-1-AP

    WB analysis of human skeletal muscle using 14600-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 14600-1-AP

    WB analysis of mouse brain using 14600-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 14600-1-AP

    WB analysis of mouse lung using 14600-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 14600-1-AP

    WB analysis of NIH/3T3 using 14600-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 14600-1-AP

    WB analysis of mouse heart using 14600-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 14600-1-AP

    WB analysis of mouse liver using 14600-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 14600-1-AP

    WB analysis of rat brain using 14600-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 14600-1-AP

    WB analysis of HSC-T6 using 14600-1-AP

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 14600-1-AP

    IP experiment of mouse brain using 14600-1-AP

    IP Result of anti-LC3 (IP:14600-1-AP, 3ug; Detection:14600-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of human gliomas using 14600-1-AP

    IHC staining of human gliomas using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 14600-1-AP

    IHC staining of human gliomas using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 14600-1-AP

    IHC staining of human breast cancer using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14600-1-AP (LC3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 14600-1-AP

    IHC staining of human breast cancer using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14600-1-AP (LC3 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse brain using 14600-1-AP

    IHC staining of mouse brain using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 14600-1-AP

    IHC staining of mouse brain using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 14600-1-AP

    IHC staining of human testis using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14600-1-AP (LC3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 14600-1-AP

    IHC staining of human testis using 14600-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14600-1-AP (LC3 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 14600-1-AP

    IF Staining of HeLa using 14600-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using LC3 antibody (14600-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 14600-1-AP

    IF Staining of U2OS using 14600-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed Chloroquine treated U2OS cells using LC3 antibody (14600-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 14600-1-AP

    IF Staining of HepG2 using 14600-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed CQ treated HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 14600-1-AP

    IF Staining of HepG2 using 14600-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed CQ treated HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 14600-1-AP

    FC experiment of HeLa using 14600-1-AP

    1X10^6 HeLa cells were stained with 0.2ug LC3 antibody (14600-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inChloroquine treated HepG2 cells, HeLa cells, mouse testis tissue, human skeletal muscle tissue, mouse heart tissue, mouse liver tissue, mouse brain tissue, mouse lung tissue, NIH/3T3 cells, HSC-T6 cells, Chloroquine treated HEK-293 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue, human testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inChloroquine treated HeLa cells, Chloroquine treated HepG2 cells, Chloroquine treated U2OS cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

14600-1-AP targets LC3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, chicken, duck, goat, monkey, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LC3 fusion protein Ag6144
Full Name microtubule-associated protein 1 light chain 3 beta
Calculated molecular weight 15 kDa
Observed molecular weight 14-18 kDa
GenBank accession numberBC067797
Gene symbol MAP1LC3B
Gene ID (NCBI) 81631
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Protocols

Product Specific Protocols
WB protocol for LC3 antibody 14600-1-APDownload protocol
IHC protocol for LC3 antibody 14600-1-APDownload protocol
IF protocol for LC3 antibody 14600-1-APDownload protocol
IP protocol for LC3 antibody 14600-1-APDownload protocol
FC protocol for LC3 antibody 14600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nanotoxicology

Silver nanoparticles induce protective autophagy via Ca2+/CaMKKβ/AMPK/mTOR pathway in SH-SY5Y cells and rat brains.

Authors - Lin Li
View Article
humanWB

J Cancer

Lyn Kinase Promotes the Proliferation of Malignant Melanoma Cells through Inhibition of Apoptosis and Autophagy via the PI3K/Akt Signaling Pathway.

Authors - Qianqian Zhang
View Article
mouseWB

Int Immunopharmacol

Protective effects of levo-tetrahydropalmatine on hepatic ischemia/reperfusion injury are mediated by inhibition of the ERK/NF-κB pathway.

Authors - Qiang Yu
View Article
mouseIF

Connect Tissue Res

Rapamycin attenuates articular cartilage degeneration by inhibiting β-catenin in a murine model of osteoarthritis.

Authors - Long Ma
View Article
humanWB

Cell Death Dis

Microglia as modulators of exosomal alpha-synuclein transmission.

Authors - Yun Xia
View Article
ratWB

FASEB J

STAT3 phosphorylation mediates high glucose-impaired cell autophagy in an HDAC1-dependent and -independent manner in Schwann cells of diabetic peripheral neuropathy.

Authors - Wei Du
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

S (Verified Customer) (12-31-2021)

I have tried three different LC3B antibodies from three different companies so far. This antibody is by far the best (cost efficient as well)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HCT116
LC3 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:14600-1-AP)
FH

YING (Verified Customer) (09-25-2021)

The antibody works very well. Distinguished LC3-I and LC3-II bands. An increase of LC3-II level in response to treatment with bafilomycin A.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: mouse cells
LC3 Antibody Western Blot validation (1:1500 dilution) in mouse cells (Cat no:14600-1-AP)
FH

Eric (Verified Customer) (03-13-2021)

Worked very well. I was able to get the LC3A/B bands on western blot using a 12.5% gel and prominent bands on a 8% gel. IF imaging worked fairly well, I used 1:100 which was likely too low so I will repeat at higher but got signal nonetheless. This performed better than some of the other LC3 antibodies we tested.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Microglia
LC3 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Microglia (Cat no:14600-1-AP)
FH

Elzbieta (Verified Customer) (09-23-2020)

The antibody works very nicely in WB. Clear signal, no aspecific bands. Our lab discarded other products and switched to this antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse liver, human HepG2 cells
FH

An (Verified Customer) (09-17-2020)

Used for IHC on zebrafish cryosections of eyes. Worked well.

  • Applications: Immunohistochemistry
FH

David (Verified Customer) (03-30-2020)

Very nice visualisation of LC3-I and LC3-II. Run on 20% gel and significant changes in response to treatment with bafilomycin A and concanamycin A.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y