LC3 Polyclonal antibody

LC3 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14600-1-AP

Synonyms

LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B



Tested Applications

Positive WB detected inChloroquine treated HepG2 cells, MCF-7 cells, HSC-T6 cells, mouse brain tissue, mouse lung tissue, NIH/3T3 cells, mouse heart tissue, mouse liver tissue, Chloroquine treated HEK-293 cells, HeLa cells, mouse testis tissue, human skeletal muscle tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue, human testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inChloroquine treated HepG2 cells, Starvation treated HEK-293 cells, HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:2500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

14600-1-AP targets LC3 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity bovine, duck, goat, human, monkey, mouse, pig, rabbit, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LC3 fusion protein Ag6144
Full Name microtubule-associated protein 1 light chain 3 beta
Calculated molecular weight 15 kDa
Observed molecular weight 14-18 kDa
GenBank accession numberBC067797
Gene symbol MAP1LC3B
Gene ID (NCBI) 81631
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Protocols

Product Specific Protocols
WB protocol for LC3 antibody 14600-1-APDownload protocol
IHC protocol for LC3 antibody 14600-1-APDownload protocol
IF protocol for LC3 antibody 14600-1-APDownload protocol
IP protocol for LC3 antibody 14600-1-APDownload protocol
FC protocol for LC3 antibody 14600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nanotoxicology

Silver nanoparticles induce protective autophagy via Ca2+/CaMKKβ/AMPK/mTOR pathway in SH-SY5Y cells and rat brains.

Authors - Lin Li
humanWB

J Cancer

Lyn Kinase Promotes the Proliferation of Malignant Melanoma Cells through Inhibition of Apoptosis and Autophagy via the PI3K/Akt Signaling Pathway.

Authors - Qianqian Zhang
mouseWB

Int Immunopharmacol

Protective effects of levo-tetrahydropalmatine on hepatic ischemia/reperfusion injury are mediated by inhibition of the ERK/NF-κB pathway.

Authors - Qiang Yu
mouseIF

Connect Tissue Res

Rapamycin attenuates articular cartilage degeneration by inhibiting β-catenin in a murine model of osteoarthritis.

Authors - Long Ma
humanWB

Cell Death Dis

Microglia as modulators of exosomal alpha-synuclein transmission.

Authors - Yun Xia
ratWB

FASEB J

STAT3 phosphorylation mediates high glucose-impaired cell autophagy in an HDAC1-dependent and -independent manner in Schwann cells of diabetic peripheral neuropathy.

Authors - Wei Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Eric (Verified Customer) (03-13-2021)

Worked very well. I was able to get the LC3A/B bands on western blot using a 12.5% gel and prominent bands on a 8% gel. IF imaging worked fairly well, I used 1:100 which was likely too low so I will repeat at higher but got signal nonetheless. This performed better than some of the other LC3 antibodies we tested.

  • Applications: Western Blot,Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Microglia
LC3 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Microglia (Cat no:14600-1-AP)
FH

Elzbieta (Verified Customer) (09-23-2020)

The antibody works very nicely in WB. Clear signal, no aspecific bands. Our lab discarded other products and switched to this antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse liver, human HepG2 cells
FH

An (Verified Customer) (09-17-2020)

Used for IHC on zebrafish cryosections of eyes. Worked well.

  • Applications: Immunohistochemistry
FH

David (Verified Customer) (03-30-2020)

Very nice visualisation of LC3-I and LC3-II. Run on 20% gel and significant changes in response to treatment with bafilomycin A and concanamycin A.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y