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  • Featured Product
  • KD/KO Validated

MCC Polyclonal antibody

MCC Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11525-1-AP

Synonyms

MCC, MCC1, mutated in colorectal cancers, Protein MCC

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MCC Polyclonal antibody (11525-1-AP)

    WB analysis of HeLa using 11525-1-AP

    WB result of MCC antibody (11525-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCC transfected HeLa cells.

  • Western Blot (WB) analysis of fetal human brain tissue using MCC Polyclonal antibody (11525-1-AP)

    WB analysis of fetal human brain using 11525-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using MCC Polyclonal antibody (11525-1-AP)

    WB analysis of L02 using 11525-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MCC Polyclonal antibody (11525-1-AP)

    WB analysis of human brain using 11525-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MCC Polyclonal antibody (11525-1-AP)

    WB analysis of mouse liver using 11525-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using MCC Polyclonal antibody (11525-1-AP)

    IP experiment of PC-3 using 11525-1-AP

    IP result of anti-MCC (IP:11525-1-AP, 3ug; Detection:11525-1-AP 1:500) with PC-3 cells lysate 5000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MCC Polyclonal antibody (11525-1-AP)

    IHC staining of human prostate cancer using 11525-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11525-1-AP (MCC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MCC Polyclonal antibody (11525-1-AP)

    IHC staining of human prostate cancer using 11525-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11525-1-AP (MCC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using MCC Polyclonal antibody (11525-1-AP)

    IF Staining of PC-3 using 11525-1-AP

    Immunofluorescent analysis of PC-3 cells using 11525-1-AP (MCC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected infetal human brain tissue, HeLa cells, human brain tissue, L02 cells, mouse liver tissue
Positive IP detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11525-1-AP targets MCC in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCC fusion protein Ag2089
Full Name mutated in colorectal cancers
Calculated Molecular Weight 829 aa, 93 kDa
Observed Molecular Weight 98 kDa, 113-118 kDa
GenBank Accession NumberBC018919
Gene Symbol MCC
Gene ID (NCBI) 4163
RRIDAB_2143796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCC (mutated in colorectal cancers) gene been previously been identified by virtue of its mutation in human colorectal tumors. As a candidate for the putative colorectal tumor suppressor gene located at 5q21, MCC protein suppresses cell proliferation a3nd the Wnt/b-catenin pathway in colorectal cancer cells. In addition, MCC is involved in cell migration via binding with Srib and b-catenin,

Protocols

Product Specific Protocols
WB protocol for MCC antibody 11525-1-APDownload protocol
IHC protocol for MCC antibody 11525-1-APDownload protocol
IF protocol for MCC antibody 11525-1-APDownload protocol
IP protocol for MCC antibody 11525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Oncogene

'MCC' protein interacts with E-cadherin and β-catenin strengthening cell-cell adhesion of HCT116 colon cancer cells.

Authors - F A Benthani
View Article
humanWB

Proteomics

Protein cross-talk in CD133+ colon cancer cells indicates activation of the Wnt pathway and upregulation of SRp20 that is potentially involved in tumorigenicity.

Authors - Corbo Claudia C
View Article
human,mouseWB,IHC,IF

Front Med (Lausanne)

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Authors - Hui Song
  • KD Validated
View Article
mouseIHC

PLoS One.

Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.

Authors - Rohrbeck A
View Article