REEP5 Polyclonal antibody

• Featured Product
• KD/KO Validated

REEP5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

48

Reviews (5)

Cat no : 14643-1-AP

Synonyms

TB2, Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1

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Validation Data Gallery

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  WB analysis using 14643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14643-1-AP

  WB result of REEP5 antibody (14643-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells.

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  WB analysis of human liver using 14643-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14643-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14643-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP (REEP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14643-1-AP

  IP result of anti-REEP5 (IP:14643-1-AP, 3ug; Detection:14643-1-AP 1:1000) with HeLa cells lysate 2400ug.

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  IHC staining of human colon cancer using 14643-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14643-1-AP (REEP5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14643-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14643-1-AP (REEP5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 14643-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (14643-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  IF Staining of HeLa using 14643-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14643-1-AP (REEP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14643-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug REEP5 Polyclonal antibody (14643-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, human liver tissue, mouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells, HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 36 publications below
• IHC: See 1 publications below
• IF: See 20 publications below
• IP: See 4 publications below

Product Information

14643-1-AP targets REEP5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey, xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: REEP5 fusion protein Ag6229
• Full Name: receptor accessory protein 5
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-21 kDa
• GenBank Accession Number: BC065926
• Gene Symbol: REEP5
• Gene ID (NCBI): 7905
• RRID: AB_2178440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

Publications

Species	Application	Title
human	WB	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human	WB	Cell; A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.; Authors - Jin Rui Liang ; KO Validated
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
human	WB	Nat Cell Biol; Combinatorial selective ER-phagy remodels the ER during neurogenesis; Authors - Melissa J Hoyer
human	WB	Nat Struct Mol Biol; Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis; Authors - Wei Zhang
human,mouse	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (05-10-2022)

Sharp and strong signal at 21 kDa with clear background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HeLa cell line

REEP5 Antibody Western Blot validation (1:250 dilution) in HeLa cell line (Cat no:14643-1-AP)

FH

Christine (Verified Customer) (11-10-2021)

Very good detection of endogenous REEP5 protein in human HEK293T and HeLa cell lines. Band at about 20 kDa (expected size for REEP5)

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Cell culture of HeLa and HEK293T

FH

Wei (Verified Customer) (09-22-2020)

The antibody gets sharp, single band on western blot.

• Applications: Western Blot
• Cell Tissue Type: Mouse heart

FH

WEI (Verified Customer) (06-29-2020)

The staining pattern is consistent with cardiomyocytes like ER localization.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Heart

FH

H (Verified Customer) (04-05-2020)

The antibody is very sensitive, such that 1 hour incubation using 1:3000 dilution worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T and HCT116

REEP5 Antibody Western Blot validation (1:3000 dilution) in HEK293T and HCT116 (Cat no:14643-1-AP)

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