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REEP5 Polyclonal antibody
REEP5 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 14643-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, human liver tissue, mouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14643-1-AP targets REEP5 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, monkey, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||REEP5 fusion protein Ag6229|
|Full Name||receptor accessory protein 5|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||18-21 kDa|
|GenBank accession number||BC065926|
|Gene ID (NCBI)||7905|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).
|Product Specific Protocols|
|WB protocol for REEP5 antibody 14643-1-AP||Download protocol|
|IHC protocol for REEP5 antibody 14643-1-AP||Download protocol|
|IF protocol for REEP5 antibody 14643-1-AP||Download protocol|
|IP protocol for REEP5 antibody 14643-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Andrea (Verified Customer) (05-10-2022)
Sharp and strong signal at 21 kDa with clear background.
Christine (Verified Customer) (11-10-2021)
Very good detection of endogenous REEP5 protein in human HEK293T and HeLa cell lines. Band at about 20 kDa (expected size for REEP5)
Wei (Verified Customer) (09-22-2020)
The antibody gets sharp, single band on western blot.
WEI (Verified Customer) (06-29-2020)
The staining pattern is consistent with cardiomyocytes like ER localization.
H (Verified Customer) (04-05-2020)
The antibody is very sensitive, such that 1 hour incubation using 1:3000 dilution worked perfectly.