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REEP5 Polyclonal antibody
REEP5 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 14643-1-AP
|Positive WB detected in||HeLa cells, human liver tissue, human brain tissue, mouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14643-1-AP targets REEP5 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||REEP5 fusion protein Ag6229|
|Full Name||receptor accessory protein 5|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||18-21 kDa|
|GenBank accession number||BC065926|
|Gene ID (NCBI)||7905|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).
|Product Specific Protocols|
|WB protocol for REEP5 antibody 14643-1-AP||Download protocol|
|IHC protocol for REEP5 antibody 14643-1-AP||Download protocol|
|IF protocol for REEP5 antibody 14643-1-AP||Download protocol|
|IP protocol for REEP5 antibody 14643-1-AP||Download protocol|
|FC protocol for REEP5 antibody 14643-1-AP||Download protocol|
|Click here to view our Standard Protocols|
ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.
Sequential formation of different layers of dystrophic neurites in Alzheimer's brains.
Mol Biol Cell
REEP3 and REEP4 determine the tubular morphology of the endoplasmic reticulum during mitosis.
TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.
Res Vet Sci
Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus.
Molecular Mechanisms of Disease Pathogenesis Differ in Krabbe Disease Variants.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (09-22-2020)
The antibody gets sharp, single band on western blot.
WEI (Verified Customer) (06-29-2020)
The staining pattern is consistent with cardiomyocytes like ER localization.
H (Verified Customer) (04-05-2020)
The antibody is very sensitive, such that 1 hour incubation using 1:3000 dilution worked perfectly.