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  • KD/KO Validated

REEP5 Polyclonal antibody

REEP5 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14643-1-AP

Synonyms

C5orf18, D5S346, DP1, Polyposis locus protein 1, Protein TB2, receptor accessory protein 5, REEP5, TB2



Tested Applications

Positive WB detected inHeLa cells, human liver tissue, human brain tissue, mouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, HeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14643-1-AP targets REEP5 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, Xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REEP5 fusion protein Ag6229
Full Name receptor accessory protein 5
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC065926
Gene symbol REEP5
Gene ID (NCBI) 7905
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

Protocols

Product Specific Protocols
WB protocol for REEP5 antibody 14643-1-APDownload protocol
IHC protocol for REEP5 antibody 14643-1-APDownload protocol
IF protocol for REEP5 antibody 14643-1-APDownload protocol
IP protocol for REEP5 antibody 14643-1-APDownload protocol
FC protocol for REEP5 antibody 14643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,monkeyWB,IF

Curr Biol

ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.

Authors - Qingzhou Chen
mouseIF

Mol Psychiatry

Sequential formation of different layers of dystrophic neurites in Alzheimer's brains.

Authors - Md Golam Sharoar
humanWB

Mol Biol Cell

REEP3 and REEP4 determine the tubular morphology of the endoplasmic reticulum during mitosis.

Authors - Darshan Kumar
  • KD Validated

Mol Cell

TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.

Authors - Heeseon An
mouseWB

Res Vet Sci

Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus.

Authors - Lingxia Li
IF

Traffic

Molecular Mechanisms of Disease Pathogenesis Differ in Krabbe Disease Variants.

Authors - Samantha J Spratley

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (09-22-2020)

The antibody gets sharp, single band on western blot.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse heart
FH

WEI (Verified Customer) (06-29-2020)

The staining pattern is consistent with cardiomyocytes like ER localization.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart
FH

H (Verified Customer) (04-05-2020)

The antibody is very sensitive, such that 1 hour incubation using 1:3000 dilution worked perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T and HCT116
REEP5 Antibody Western Blot validation (1:3000 dilution) in HEK293T and HCT116 (Cat no:14643-1-AP)