SNX27 Polyclonal antibody

SNX27 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16329-1-AP

Synonyms

KIAA0488, MRT1, MY014, SNX27, Sorting nexin 27, sorting nexin family member 27



Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue
Positive IHC detected inhuman cervical cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 16329-1-AP is SNX27 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX27 fusion protein Ag9447
Full Name sorting nexin family member 27
Calculated molecular weight 61 kDa, 60 kDa, 52 kDa, 28 kDa
Observed molecular weight 52-60 kDa
GenBank accession numberBC012184
Gene symbol SNX27
Gene ID (NCBI) 81609
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sorting nexin 27 (SNX27), a PDZ domain-containing protein, belongs to the sorting nexin family of proteins. SNX27 promotes recycling of internalized transmembrane proteins from endosomes to the plasma membrane by linking PDZ-dependent cargo recognition to retromer-mediated transport (PMID: 21602791; 23563491). It has been shown that SNX27 deficiency contributes to the synaptic and cognitive deficits by modulating glutamate receptor recycling in Down's syndrome (PMID: 23524343). SNX27 has also been proposed to have a role in regulating β-amyloid (Aβ) generation by modulating γ-secretase activity, which is a molecular mechanism for Aβ-dependent pathogenesis in both Down's syndrome and Down's syndrome (PMID: 25437557).

Protocols

Product Specific Protocols
WB protocol for SNX27 antibody 16329-1-APDownload protocol
IHC protocol for SNX27 antibody 16329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit.

Authors - Xuan Ling Hilary Yong
humanWB

J Cell Biol

Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.

Authors - Boris Simonetti