MAD2L1 Polyclonal antibody

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  WB analysis using 10337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10337-1-AP

  WB result of MAD2L1 antibody (10337-1-AP, 1:500) with si-control and si-MAD2L1 transfected HEK293 cells.

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  WB analysis of rat liver using 10337-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10337-1-AP

  IP result of anti-MAD2L1 (IP:10337-1-AP, 3ug; Detection:10337-1-AP 1:500) with HEK-293 cells lysate 1800ug.

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  IHC staining of mouse ovary using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 25x lens).

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  IHC staining of human liver cancer using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 10337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAD2L1 antibody (10337-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HEK-293 using 10337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

MAD2L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10337-1-AP

Publications(29)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2 like protein 1, MAD2, HSMAD2

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Tested Applications

• Positive WB detected in: Daudi cells, rat liver tissue, HEK-293 cells, HepG2 cells, Raji cells, THP-1 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse ovary tissue, human liver cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 20 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Product Information

10337-1-AP targets MAD2L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAD2L1 fusion protein Ag0307
• Full Name: MAD2 mitotic arrest deficient-like 1 (yeast)
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC000356
• Gene Symbol: MAD2L1
• Gene ID (NCBI): 4085
• RRID: AB_2139365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13257
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

Publications

Species	Application	Title
	WB	Sci Adv; The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.; Authors - Changyin Zhou
human	WB,IF	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
	IF	J Cell Biol; Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.; Authors - Tianyu Wu
human	WB	Oncogene; KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.; Authors - Zhi-Chuan Zhu
	WB,IHC	Cancer Res; Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.; Authors - He Feng F
		Cancer Res; MPHOSPH1: a potential therapeutic target for hepatocellular carcinoma.; Authors - Xinran Liu