• Featured Product
  • KD/KO Validated

MAD2L1 Polyclonal antibody

MAD2L1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10337-1-AP


HSMAD2, MAD2, MAD2 like protein 1, MAD2L1

Tested Applications

Positive WB detected inDaudi cells, rat liver tissue, HEK-293 cells, HepG2 cells, Raji cells, THP-1 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10337-1-AP targets MAD2L1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAD2L1 fusion protein Ag0307
Full Name MAD2 mitotic arrest deficient-like 1 (yeast)
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC000356
Gene Symbol MAD2L1
Gene ID (NCBI) 4085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.


Product Specific Protocols
WB protocol for MAD2L1 antibody 10337-1-APDownload protocol
IHC protocol for MAD2L1 antibody 10337-1-APDownload protocol
IF protocol for MAD2L1 antibody 10337-1-APDownload protocol
IP protocol for MAD2L1 antibody 10337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.

Authors - Changyin Zhou

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan

J Cell Biol

Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.

Authors - Tianyu Wu


KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Authors - Zhi-Chuan Zhu

Cancer Res

Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.

Authors - He Feng F

Cancer Res

MPHOSPH1: a potential therapeutic target for hepatocellular carcinoma.

Authors - Xinran Liu