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MAD2L1 Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 10337-1-AP

Featured Product KD/KO validated

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Con: 31 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

MAD2L1 Antibody WB HEK-293 cells 10337-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10337-1-AP(MAD2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

MAD2L1 Antibody IHC human liver cancer tissue 10337-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP(MAD2L1 antibody) at dilution of 1:50 (under 25x lens)

MAD2L1 Antibody WB HEK-293 cells 10337-1-AP

WB result of MAD2L1 antibody (10337-1-AP, 1:500) with si-control and si-MAD2L1 transfected HEK293 cells.

MAD2L1 Antibody IP HEK-293 cells 10337-1-AP

IP Result of anti-MAD2L1 (IP:10337-1-AP, 3ug; Detection:10337-1-AP 1:500) with HEK-293 cells lysate 1800ug.

MAD2L1 Antibody IF HEK-293 cells 10337-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed HEK-293 cells using 10337-1-AP(MAD2L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, HEK-293
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human liver cancer tissue, human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HEK-293 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MAD2L1 fusion protein Ag0307
Full name:
MAD2 mitotic arrest deficient-like 1 (yeast)
Calculated molecular weight:
24 kDa
Observed molecular weight:
25-30 kDa
GenBank accession number:
BC000356
Gene ID (NCBI):
4085
Gene symbol
MAD2L1
Synonyms
HSMAD2, MAD2, MAD2 like protein 1, MAD2L1

Background

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.


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