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Beta Catenin Polyclonal antibody
Beta Catenin Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 51067-2-AP
|Positive WB detected in||C6 cells, NIH/3T3 cells, A431 cells, human placenta tissue, HeLa cells, HEK-293 cells, mouse liver tissue, mouse lung tissue, SH-SY5Y cells, MCF-7 cells, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human colon cancer tissue, human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells, T-47D cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
51067-2-AP targets Beta Catenin in WB, IP, IHC, IF, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||bovine, canine, goat, hamster, human, mouse, rat, swine, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Full Name||catenin (cadherin-associated protein), beta 1, 88kDa|
|Calculated molecular weight||781 aa, 86 kDa|
|Observed molecular weight||92 kDa|
|GenBank accession number||BC058926|
|Gene ID (NCBI)||1499|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
|Product Specific Protocols|
|WB protocol for Beta Catenin antibody 51067-2-AP||Download protocol|
|IHC protocol for Beta Catenin antibody 51067-2-AP||Download protocol|
|IF protocol for Beta Catenin antibody 51067-2-AP||Download protocol|
|IP protocol for Beta Catenin antibody 51067-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Alzbeta (Verified Customer) (03-13-2019)
This antibody was used for IF staining of beta-catenin in primary neonatal rat cardiomyocyte culture. The antibody worked well after both ice cold-methanol or 4% PFA fixation, followed by the 3%BSA block. The incubation with primary (b-catenin) and secondary antibody (aRabbit-Alexa Fluor 488 Plus) lasted 1 hr at room temperature.