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  • KD/KO Validated

MICAL1 Antibody

MICAL1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 14818-1-AP

Synonyms

MICAL, MICAL 1, MICAL1, NICAL



Tested Applications

Positive WB detected inJurkat cells, human brain tissue, mouse lung tissue, HeLa cells, HEK-293 cells, T-47D cells
Positive IP detected inHeLa cells, T-47D cells
Positive IHC detected inhuman lung tissue, human heart tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14818-1-AP targets MICAL1 in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MICAL1 fusion protein Ag6578
Full Name microtubule associated monoxygenase, calponin and LIM domain containing 1
Calculated molecular weight 118 kDa
Observed molecular weight 120 kDa
GenBank accession number BC052983
Gene symbol MICAL1
Gene ID (NCBI) 64780
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.

Protocols

Product Specific Protocols
WB protocol for MICAL1 antibody 14818-1-APDownload protocol
IHC protocol for MICAL1 antibody 14818-1-APDownload protocol
IF protocol for MICAL1 antibody 14818-1-APDownload protocol
IP protocol for MICAL1 antibody 14818-1-APDownload protocol
FC protocol for MICAL1 antibody 14818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Pharmacol

NEDD9 Facilitates Hypoxia-Induced Gastric Cancer Cell Migration via MICAL1 Related Rac1 Activation.

Authors - Shuo Zhao
  • KD Validated

Methods Mol Biol

Podocyte Shape Regulation by Semaphorin 3A and MICAL-1.

Authors - Alda Tufro
humanWB,IF

BMC Cancer

MICAL1 controls cell invasive phenotype via regulating oxidative stress in breast cancer cells.

Authors - Wenjie Deng
  • KD Validated
humanWB

J Cell Mol Med

MICAL1 facilitates breast cancer cell proliferation via ROS-sensitive ERK/cyclin D pathway.

Authors - Wenjie Deng
  • KD Validated
humanWB,IF

Oncotarget

NEDD9 stimulated MMP9 secretion is required for invadopodia formation in oral squamous cell carcinoma.

Authors - Stéphane Grauzam
  • KD Validated
humanWB

J Cell Sci

Differential regulation of actin microfilaments by human MICAL proteins.

Authors - Giridharan Sai Srinivas Panapakkam SS
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen Cheah (06-03-2019)

Shows band at where MICAL1 is expected but is faint. This image is a merge image of the membrane exposed for 120s and a stain free image to visualize the molecular weight. Even with long exposure, the band is faint.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
MICAL1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:14818-1-AP)