MT1-MMP Antibody

MT1-MMP Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14552-1-AP

Synonyms

Matrix metalloproteinase 14, MMP 14, MMP X1, MMP14, MT MMP 1, MT1 MMP, MT1MMP, MT1-MMP, MTMMP1



Tested Applications

Positive WB detected inHepG2 cells, A549 cells, COLO 320 cells, human colon tissue
Positive IF detected inHepG2 cells, COLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14552-1-AP targets MT1-MMP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MT1-MMP fusion protein Ag5965
Full Name matrix metallopeptidase 14 (membrane-inserted)
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa,45-50 kDa
GenBank accession number BC064803
Gene symbol MMP14
Gene ID (NCBI) 4323
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP14, also named as MT1-MMP, is a key matrix metalloproteinase (MMP) family member which plays a crucial role in tumor growth, invasion and metastasis. MT1-MMP is a cell membrane-bound proteinase, and it enhances degradation of collagen IV, a major component of the basement membrane, by forming a complex with tissue inhibitor of metalloproteinase-2 (TIMP-2) to activate pro-MMP-2. MT1-MMP can influence venous invasion, intrahepatic metastasis , and patient outcome in hepatocellular carcinoma (HCC). MT1-MMP was reported to be present in centromere and could lead to chromosome instability in MDCK cells, indicating that MT1-MMP may have more novel functions in the intracellular compartments. In western blotting, pro-MMP14 (65 kDa) and MMP14 (51 kDa) bands showed with the truncated MMP14 (45, 42, 35, 20 kDa) forms (PMID:12097451).

Protocols

Product Specific Protocols
WB protocol for MT1-MMP antibody 14552-1-APDownload protocol
IF protocol for MT1-MMP antibody 14552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

J Cell Physiol

MT1-MMP downregulation via the PI3K/Akt signaling pathway is required for the mechanical stretching-inhibited invasion of bone-marrow-derived mesenchymal stem cells.

Authors - Xiaorong Fu
humanWB

Reprod Sci

SPOCK2 Affects the Biological Behavior of Endometrial Cancer Cells by Regulation of MT1-MMP and MMP2.

Authors - Fang Ren
humanWB

Mol Ther Nucleic Acids

Knockdown of USF1 Inhibits the Vasculogenic Mimicry of Glioma Cells via Stimulating SNHG16/miR-212-3p and linc00667/miR-429 Axis.

Authors - Di Wang
humanWB

Cancers (Basel)

NAB 2-Expressing Cancer-Associated Fibroblast Promotes HNSCC Progression.

Authors - So-Young Choi
humanWB,IHC

Eur Rev Med Pharmacol Sci

MMP-14 aggravates onset of severe preeclampsia by mediating soluble endoglin release.

Authors - X-H Zhang
humanWB

Biomed Res Int

VEGF Induce Vasculogenic Mimicry of Choroidal Melanoma through the PI3k Signal Pathway.

Authors - Xiaoyan Xu