|Positive WB detected in||HEK-293 cells, C2C12 cells, HeLa cells, Jurkat cells, MCF-7 cells, C6 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human heart tissue, human gliomas tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:3000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22117-1-AP is MYPT1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MYPT1 fusion protein Ag17496|
|Full Name||protein phosphatase 1, regulatory (inhibitor) subunit 12A|
|Calculated molecular weight||1030 aa, 115 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC111752|
|Gene ID (NCBI)||4659|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization. Several transcript variants encoding different isoforms have been found for this gene.
|Product Specific Protocols|
|WB protocol for MYPT1 antibody 22117-1-AP||Download protocol|
|IHC protocol for MYPT1 antibody 22117-1-AP||Download protocol|
|IF protocol for MYPT1 antibody 22117-1-AP||Download protocol|
|IP protocol for MYPT1 antibody 22117-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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