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NCAM1/CD56 Polyclonal antibody

NCAM1/CD56 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14255-1-AP

Synonyms

CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1



Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inhuman lung cancer tissue, Insulinoma tissue, human colon tissue, human tonsillitis tissue, mouse brain tissue, human gliomas tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:20000
Sample-dependent, check data in validation data gallery

Product Information

14255-1-AP targets NCAM1/CD56 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCAM1/CD56 fusion protein Ag5528
Full Name neural cell adhesion molecule 1
Calculated molecular weight 95 kDa
Observed molecular weight 120 kDa, 140 kDa, 180 kDa
GenBank accession numberBC047244
Gene symbol NCAM1
Gene ID (NCBI) 4684
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurones, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

Protocols

Product Specific Protocols
WB protocol for NCAM1/CD56 antibody 14255-1-APDownload protocol
IHC protocol for NCAM1/CD56 antibody 14255-1-APDownload protocol
FC protocol for NCAM1/CD56 antibody 14255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Br J Cancer

Interferon-alpha promotes immunosuppression through IFNAR1/STAT1 signalling in head and neck squamous cell carcinoma.

Authors - Hailong Ma
pigIHC

PLoS One

Food hypersensitivity-induced chronic gastrointestinal inflammation in a non-human primate model of diet-induced obesity.

Authors - Tomris Mustafa
humanIHC

Cell Mol Gastroenterol Hepatol

Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice.

Authors - Sohji Nishina
mouseIF

Nat Med

Selective modulation of the androgen receptor AF2 domain rescues degeneration in spinal bulbar muscular atrophy.

Authors - Nisha M Badders
humanIF

PLoS One

Flow Cytometric Immunophenotyping Is Sensitive for the Early Diagnosis of De Novo Aggressive Natural Killer Cell Leukemia (ANKL): A Multicenter Retrospective Analysis.

Authors - Yi Li
mouseIHC

PLoS One

Combined B, T and NK Cell Deficiency Accelerates Atherosclerosis in BALB/c Mice.

Authors - Fei Cheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Toni (Verified Customer) (02-28-2019)

IP conditions: 1ug of rbt anti-NCAM1 was added to 100ug human cortex homogenate (0.5ug/ul) in Tris-sucrose buffer with 1% SDS and 1% Triton X100 then incubated rotating ON at 4C. Sample was then added to 50ul TBST-washed sheep anti-rabbit magnetic beads and incubated rotating 3hr at 4C. Following 4 TBST washes, bound proteins were eluted with 30ul of elution buffer containing SDS and BME. WB conditions: Samples subjected to SDS-PAGE on 4-12% Bis-Tris gels followed by semi-dry transfer to nitrocellulose membranes using standard conditions. Membranes were blocked for 1hr at RT in 50% LiCor Odyssey blocking buffer (TBS) then probed with either 1:5000 (v/v) rabbit anti-NCAM1 (Proteintech) or 1:1000 (v/v) goat anti-NCAM1 (R&D Systems) in 50% LiCor Odyssey blocking buffer (TBS + 0.05% Tween-20) ON at 4C. Following TBS+ 0.1% Tween-20 washes, membranes were incubated with the appropriate IR-dye labeled secondary antibody for 1hr at RT, TBST washed, then scanned. *you have my permission to edit the comments above and crop, but not alter, the image provided if you wish to remove reference to an antibody from another company.

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:5000 in 50% LiCor Odyssey Blocking Buffer (TBS+0.1%Tween 20)
  • Cell Tissue Type: postmortem human cortex
NCAM1/CD56 Antibody Western Blot,Immunoprecipitation, validation (1:5000 in 50% LiCor Odyssey Blocking Buffer (TBS+0.1%Tween 20) dilution) in postmortem human cortex (Cat no:14255-1-AP)