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NDUFB6 Polyclonal antibody
NDUFB6 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16037-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells, MCF-7 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
Product Information
16037-1-AP targets NDUFB6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NDUFB6 fusion protein Ag8908 |
| Full Name | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa |
| Calculated Molecular Weight | 128 aa, 15 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC009801 |
| Gene Symbol | NDUFB6 |
| Gene ID (NCBI) | 4712 |
| RRID | AB_2235901 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NDUFB6, also named as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 or Complex I-B17, is a 128 amino acid protein, which belongs to the complex I NDUFB6 subunit family. NDUFB6 localizes in the Mitochondrion inner membrane and is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFB6 antibody 16037-1-AP | Download protocol |
| IHC protocol for NDUFB6 antibody 16037-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.
| ||
Redox Biol Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia. | ||
J Nutr Biochem Deficiency of β-carotene oxygenase 2 induces mitochondrial fragmentation and activates the STING-IRF3 pathway in the mouse hypothalamus. | ||
Mitochondrion MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism. | ||
FEBS Lett Apoptosis-inducing factor is a target gene of C/EBPα and participates in adipocyte differentiation. |
