NDUFS7 Polyclonal antibody

NDUFS7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 15728-1-AP

Synonyms

CI 20KD, Complex I 20kD, MY017, NDUFS7, PSST, PSST subunit

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Validation Data Gallery

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  WB analysis of A549 using 15728-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15728-1-AP (NDUFS7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15728-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15728-1-AP (NDUFS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15728-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15728-1-AP (NDUFS7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15728-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15728-1-AP (NDUFS7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15728-1-AP

  Immunofluorescent analysis of Hela cells, using NDUFS7 antibody 15728-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A549 cells, human brain tissue, Hela cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

15728-1-AP targets NDUFS7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFS7 fusion protein Ag8471
• Full Name: NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase)
• Calculated Molecular Weight: 213 aa, 24 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC005954
• Gene Symbol: NDUFS7
• Gene ID (NCBI): 374291
• RRID: AB_2149029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFS7(NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial) protein is one of the most conserved subunits of mitochondrial respiratory chain complex I and plays a central role in the interaction with the electron acceptor ubiquinone and in the proton-translocating mechanism(PMID:15269216).Defects in NDUFS7 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

Publications

Species	Application	Title
mouse	WB	Cell Res; NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.; Authors - Tingting Hou
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	Cell Chem Biol; Metabolic targeting of cancer by a ubiquinone uncompetitive inhibitor of mitochondrial complex I.; Authors - Shashi Jain
mouse	WB	Arch Toxicol; SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice; Authors - Jia-Wen Chen
human	IF	Mol Cell Biol; TRMT1-catalyzed tRNA modifications are required for redox homeostasis to ensure proper cellular proliferation and oxidative stress survival.; Authors - Joshua M Dewe
human	WB	FEBS J; Human brain proteins showing neuron-specific interactions with γ-secretase.; Authors - Mitsuhiro Inoue