NDUFA5 Polyclonal antibody

NDUFA5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 16640-1-AP

Synonyms

B13, CI 13KD B, Complex I 13kD B, Complex I subunit B13, NDUFA5, NUFM, UQOR13

---

Validation Data Gallery

• 

  WB analysis of human brain using 16640-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16640-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16640-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16640-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16640-1-AP (NDUFA5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 16640-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16640-1-AP (NDUFA5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16640-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16640-1-AP (NDUFA5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16640-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16640-1-AP (NDUFA5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells, human heart tissue, rat liver tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below

Product Information

16640-1-AP targets NDUFA5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFA5 fusion protein Ag9995
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC000813
• Gene Symbol: NDUFA5
• Gene ID (NCBI): 4698
• RRID: AB_2251270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFA5, also named as CI-13kD-B and NDUA5, belongs to the complex I NDUFA5 subunit family. It is accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which functions in the transfer of electrons from NADH to the respiratory chain. NDUFA5,an enrichment of mitochondrial protein, can be used as a mitochondrial marker protein(PMID:21360670).

Publications

Species	Application	Title
human	WB	Mol Cell; Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria; Authors - Sabine Poerschke
human	WB	EMBO Rep; MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.; Authors - Cong Wang
human	WB	Cancer Lett; PMID: 29906487; Authors - Linhua Lan
human	WB	J Mol Biol; COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria.; Authors - David Pacheu-Grau
human	WB	Proteomics; 2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time course-dependent remodelling in response to 14 consecutive days of endurance exercise training.; Authors - Egan Brendan B
human	WB	Evid Based Complement Alternat Med; Mechanistic Study of the Phytocompound, 2- β -D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches.; Authors - Jeng-Yuan Shiau