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NDUFV1 Polyclonal antibody

NDUFV1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 11238-1-AP

Synonyms

CI 51kD, Complex I 51kD, NDUFV1, UQOR1



Tested Applications

Positive WB detected inA431 cells, human brain tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11238-1-AP targets NDUFV1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFV1 fusion protein Ag1764
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC015645
Gene symbol NDUFV1
Gene ID (NCBI) 4723
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

Protocols

Product Specific Protocols
WB protocol for NDUFV1 antibody 11238-1-APDownload protocol
IHC protocol for NDUFV1 antibody 11238-1-APDownload protocol
IP protocol for NDUFV1 antibody 11238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO Rep

CLPP deficiency protects against metabolic syndrome but hinders adaptive thermogenesis.

Authors - Christina Becker
humanWB

Front Genet

Novel MT-ND Gene Variants Causing Adult-Onset Mitochondrial Disease and Isolated Complex I Deficiency.

Authors - Yi Shiau Ng
humanWB

Am J Transl Res

Fenofibrate-induced mitochondrial dysfunction and metabolic reprogramming reversal: the anti-tumor effects in gastric carcinoma cells mediated by the PPAR pathway.

Authors - Lulu Chen
mouseWB

J Neurochem

Complex I specific increase in superoxide formation and respiration rate by PrP-null mouse brain mitochondria.

Authors - Paterson Andrew W J AW
pigWB

Sci Rep

Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes.

Authors - Jing Wen
  • KD Validated
mouseWB

J Cell Physiol

Glucose metabolism during in vitro maturation of mouse oocytes: An study using RNA interference.

Authors - Hong-Li Xie