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TTF1; NKX2-1 Polyclonal antibody

TTF1; NKX2-1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21777-1-AP

Synonyms

BHC, Homeobox protein Nkx 2.1, NK 2, NK2 homeobox 1, NKX2 1, NKX2.1, NKX2-1, NKX2A, TEBP, Thyroid nuclear factor 1, Thyroid transcription factor 1, TITF1, TTF 1, TTF1, TTF1; NKX2-1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of HeLa using 21777-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (NKX2-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of HeLa using 21777-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (NKX2-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of mouse lung using 21777-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of mouse brain using 21777-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of A549 using 21777-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of rat brain using 21777-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, rat brain tissue, A549 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21777-1-AP targets TTF1; NKX2-1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name NK2 homeobox 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 41-45 kDa
GenBank Accession NumberNM_003317
Gene Symbol NKX2-1
Gene ID (NCBI) 7080
RRIDAB_2878919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NKX2-1, also named as NKX2A, TITF1 and TTF1, belongs to the NK-2 homeobox family. It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype. It may play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). The antibody is specific to NKX2-1.

Protocols

Product Specific Protocols
WB protocol for TTF1; NKX2-1 antibody 21777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Environ Toxicol Pharmacol

Role of estrogen receptors in thyroid toxicity induced by mono (2-ethylhexyl) phthalate via endoplasmic reticulum stress: An in vitro mechanistic investigation

Authors - Qi Xu
View Article