Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, mouse lung tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21777-1-AP targets TTF1/NKX2-1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | NK2 homeobox 1 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | NM_003317 |
| Gene Symbol | TTF1 |
| Gene ID (NCBI) | 7080 |
| RRID | AB_2878919 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43699 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NKX2-1, also named as NKX2A, TITF1 and TTF1, belongs to the NK-2 homeobox family. It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype. It may play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). The antibody is specific to NKX2-1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TTF1/NKX2-1 antibody 21777-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TTF1/NKX2-1 Polyclonal antibody (Cat# 21777-1-AP) has 6 total citations. These appear in Cancer Research, Phytotherapy Research, Journal of Genetics and Genomics, Environmental Pollution, Environmental Toxicology and Pharmacology, and Toxicology and Applied Pharmacology. Research fields span lung cancer immunotherapy, macrophage polarization in lung adenocarcinoma, pulmonary lipid homeostasis, thyroid endocrine disruption, estrogen receptor-mediated thyroid toxicity, and prenatal amoxicillin effects on alveolar development.
| Species | Application | Title |
|---|---|---|
Cancer Res Loss of CDKN2A Enhances the Efficacy of Immunotherapy in EGFR Mutant Non-Small Cell Lung Cancer | ||
Phytother Res Chlorogenic Acid Regulates Macrophage Polarization in LUAD by Inhibiting Taurodeoxycholic Acid Metabolism to Block the TGR5/STAT3 Signaling Pathway. | ||
J Genet Genomics Tapt1 deficiency in mice impairs pulmonary lipid homeostasis and normal postnatal respiration by targeting ABCA3 for autophagy-lysosomal degradation. | ||
Environ Pollut PFOA substitute GenX at a sub-cytotoxic level exhibits thyroid-disrupting effects by inhibiting the expression of Thyroid Transcription Factor 1 | ||
Environ Toxicol Pharmacol Role of estrogen receptors in thyroid toxicity induced by mono (2-ethylhexyl) phthalate via endoplasmic reticulum stress: An in vitro mechanistic investigation | ||
Toxicol Appl Pharmacol Prenatal amoxicillin exposure impairs mouse alveolar development via Tet3-mediated hypo-hydroxymethylation of Nkx2-1 promoter. |
