NOTCH3 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 55114-1-AP

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Freight/Packing

Con: 28 μg/150 μl

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Species specificity:
human, mouse

Positive WB detected in:
COLO 320 cells, A2780 cells, HeLa cells, U2OS cells

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human lung cancer tissue, human breast cancer tissue, human kidney tissue, human lung tissue, mouse brain tissue

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
Notch homolog 3 (Drosophila)

Calculated molecular weight:
244 kDa

Observed molecular weight:
250-260 kDa, 100 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
NOTCH3

Synonyms
CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3
Background

NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).


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