Product Information
CL488-55114 targets NOTCH3 in applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Notch homolog 3 (Drosophila) |
| Calculated Molecular Weight | 244 kDa |
| Observed Molecular Weight | 250-260 kDa, 100 kDa |
| GenBank Accession Number | NM_000435 |
| Gene Symbol | NOTCH3 |
| Gene ID (NCBI) | 4854 |
| RRID | AB_3672854 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UM47 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).
Publications
What published studies show
CL488-55114 is a NOTCH3 Polyclonal antibody conjugated with 488 Green. It has a total of 1 citation. The citation appears in the International Journal of Oncology (Int J Oncol). The associated research field is oncology, specifically focusing on epithelial-mesenchymal transition (EMT) and cancer stemness in non-small cell lung cancer, mediated by Notch3 and TGF-β1 signaling.
| Species | Application | Title |
|---|---|---|
Int J Oncol Notch3 mediated TGF‑β1 activation enhances epithelial‑mesenchymal transition and cancer stemness in non‑small lung cancer |
