NOX3-Specific Polyclonal antibody

NOX3-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20065-1-AP

Synonyms

GP91 3, gp91phox homolog 3, Mitogenic oxidase 2, MOX 2, MOX2, NADPH oxidase 3, NOX3, NOX3-Specific



Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

20065-1-AP targets NOX3-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name NADPH oxidase 3
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberNM_015718
Gene Symbol NOX3
Gene ID (NCBI) 50508
RRIDAB_10733223
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOX3 is a member of NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. NOX3 plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. The antibody is specific to NOX3.

Protocols

Product Specific Protocols
WB protocol for NOX3-Specific antibody 20065-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Antioxidants (Basel)

Distinction between 2'- and 3'-Phosphate Isomers of a Fluorescent NADPH Analogue Led to Strong Inhibition of Cancer Cells Migration.

Authors - Raoul Manuel
mouseWB

Front Pharmacol

Nox4 Promotes RANKL-Induced Autophagy and Osteoclastogenesis via Activating ROS/PERK/eIF-2α/ATF4 Pathway

Authors - Jing Sun
humanWB

Onco Targets Ther

Brevilin A Inhibits STAT3 Signaling and Induces ROS-Dependent Apoptosis, Mitochondrial Stress and Endoplasmic Reticulum Stress in MCF-7 Breast Cancer Cells.

Authors - Muhammad Zubair Saleem
humanWB

Biomedicines

Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects

Authors - Wenfeng Hu
mouseWB

J Leukoc Biol

GKT137831 in combination with adipose-derived stem cells alleviates high glucose-induced inflamm-aging and improves diabetic wound healing

Authors - Yunxian Dong
humanWB

Am J Physiol Lung Cell Mol Physiol

Antigen stasis and airway nitrosative stress in human primary ciliary dyskinesia

Authors - Benjamin Gaston