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Neuropilin 1 Antibody 2 Publications

Mouse Monoclonal| Catalog number: 60067-1-Ig |CloneNo.: 2H3F6

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Con: 150 μg/150 μl

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Tested Applications
Applications:
ELISA, WB, IHC, IF
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Images See all

60067-1-Ig;human brain tissue

human brain tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig( Neuropilin 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours

60067-1-Ig;human embronic stem cells

Confocal immunofluorescent analysis of human embronic stem cells with 60067-1-Ig at dilution of 1:100. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml. This photo is the merge of PE and DAPI. (40X)

60067-1-Ig;human kidney tissue

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60067-1-Ig( Neuropilin 1 antibody at dilution of 1:200 (under 10x lens).

60067-1-Ig;human kidney tissue

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60067-1-Ig( Neuropilin 1 antibody at dilution of 1:200 (under 40x lens).

60067-1-Ig;SH-SY5Y cells

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed SH-SY5Y cells using 60067-1-Ig(Neuropilin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, pig
Positive WB detected in:
human brain tissue, fetal human brain tissue, human plasma tissue, pig brain tissue
Positive IHC detected in:
human kidney tissue, human gliomas tissue, human kidney tissue, human placenta tissue
Positive IF detected in:
SH-SY5Y cells, human embronic stem cells
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information

Source:
Mouse
Purification method:
Caprylic acid/ammonium sulfate precipitation
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Neuropilin 1 fusion protein ag0931
Full name:
neuropilin 1
Calculated molecular weight:
103 kDa
Observed molecular weight:
130 kDa
GenBank accession number:
BC007737
Gene ID (NCBI):
8829
Gene symbol
NRP1
Synonyms
BDCA4, CD304, DKFZp686A03134, DKFZp781F1414, neuropilin 1, NRP, NRP1, VEGF165R
Background

Neuropilin-1 (NRP1) is a transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.


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