Back

Neuropilin 1 Antibody 3 Publications

Mouse Monoclonal| Catalog number: 60067-1-Ig |CloneNo.: 2H3F6

  • Print page
  • Download PDF

Be the first to review this product

-_-

Freight/Packing

Con: 150 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Related Primary Antibodies
Special offer: Add control/tag antibody for $99
Load more

Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

Neuropilin 1 Antibody WB human brain tissue 60067-1-Ig

human brain tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig( Neuropilin 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours

Neuropilin 1 Antibody IF human embronic stem cells 60067-1-Ig

Confocal immunofluorescent analysis of human embronic stem cells with 60067-1-Ig at dilution of 1:100. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml. This photo is the merge of PE and DAPI. (40X)

Neuropilin 1 Antibody IHC human kidney tissue 60067-1-Ig

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60067-1-Ig( Neuropilin 1 antibody at dilution of 1:200 (under 10x lens).

Neuropilin 1 Antibody IHC human kidney tissue 60067-1-Ig

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60067-1-Ig( Neuropilin 1 antibody at dilution of 1:200 (under 40x lens).

Neuropilin 1 Antibody IF SH-SY5Y cells 60067-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed SH-SY5Y cells using 60067-1-Ig(Neuropilin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, pig
Positive WB detected in:
human brain tissue, fetal human plasma tissue, pig brain tissue
Positive IHC detected in:
human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
SH-SY5Y cells, human embronic stem cells
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Caprylic acid/ammonium sulfate precipitation
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Neuropilin 1 fusion protein Ag0931
Full name:
neuropilin 1
Calculated molecular weight:
103 kDa
Observed molecular weight:
130 kDa
GenBank accession number:
BC007737
Gene ID (NCBI):
8829
Gene symbol
NRP1
Synonyms
BDCA4, CD304, neuropilin 1, NRP, NRP1, VEGF165R

Background

Neuropilin-1 (NRP1) is a a 130–140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.


Back
to top