Neuropilin 1 Monoclonal antibody

Neuropilin 1 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2H3F6

Cat no : 60067-1-Ig

Synonyms

BDCA4, CD304, neuropilin 1, NRP, NRP1, VEGF165R



Tested Applications

Positive WB detected inhuman placenta tissue, human plasma, fetal human brain tissue, pig brain tissue
Positive IF detected inSH-SY5Y cells, human embronic stem cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60067-1-Ig targets Neuropilin 1 in WB, IF, FC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, Xenopus
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Neuropilin 1 fusion protein Ag0931
Full Name neuropilin 1
Calculated molecular weight 103 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC007737
Gene symbol NRP1
Gene ID (NCBI) 8829
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuropilin-1 (NRP1) is a a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.

Protocols

Product Specific Protocols
WB protocol for Neuropilin 1 antibody 60067-1-IgDownload protocol
IF protocol for Neuropilin 1 antibody 60067-1-IgDownload protocol
FC protocol for Neuropilin 1 antibody 60067-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a.

Authors - Lansheng Zhang
humanWB

Am J Med Genet A

NRP1 haploinsufficiency predisposes to the development of Tetralogy of Fallot.

Authors - Ivan Duran
humanIF

Cell Death Dis

Cdc42 subcellular relocation in response to VEGF/NRP1 engagement is associated with the poor prognosis of colorectal cancer.

Authors - Li-Li Ma
humanWB

Mol Cell Biochem

miR-338 suppresses the growth and metastasis of OSCC cells by targeting NRP1.

Authors - Chunxi Liu
humanIF

Am J Cancer Res

CMTM6 promotes cell proliferation and invasion in oral squamous cell carcinoma by interacting with NRP1.

Authors - Yang Zheng
human

J Pathol

Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma.

Authors - Eric Morin