Neuropilin 1 Monoclonal antibody
Neuropilin 1 Monoclonal Antibody for FC, IF, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig and More (1)
Applications
WB, IF, FC,ELISA
Conjugate
Unconjugated
CloneNo.
2H3F6
Cat no : 60067-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human placenta tissue, human plasma, fetal human brain tissue, pig brain tissue |
Positive IF detected in | SH-SY5Y cells, human embronic stem cells |
Positive FC detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
IF | See 4 publications below |
Product Information
60067-1-Ig targets Neuropilin 1 in WB, IF, FC,ELISA applications and shows reactivity with human, pig samples.
Tested Reactivity | human, pig |
Cited Reactivity | human, Xenopus |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Neuropilin 1 fusion protein Ag0931 |
Full Name | neuropilin 1 |
Calculated molecular weight | 103 kDa |
Observed molecular weight | 130 kDa |
GenBank accession number | BC007737 |
Gene symbol | NRP1 |
Gene ID (NCBI) | 8829 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Caprylic acid/ammonium sulfate precipitation |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neuropilin-1 (NRP1) is a a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Neuropilin 1 antibody 60067-1-Ig | Download protocol |
IF protocol for Neuropilin 1 antibody 60067-1-Ig | Download protocol |
FC protocol for Neuropilin 1 antibody 60067-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomed Pharmacother RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a. | ||
Am J Med Genet A NRP1 haploinsufficiency predisposes to the development of Tetralogy of Fallot. | ||
Cell Death Dis Cdc42 subcellular relocation in response to VEGF/NRP1 engagement is associated with the poor prognosis of colorectal cancer. | ||
Mol Cell Biochem miR-338 suppresses the growth and metastasis of OSCC cells by targeting NRP1. | ||
Am J Cancer Res CMTM6 promotes cell proliferation and invasion in oral squamous cell carcinoma by interacting with NRP1. | ||
J Pathol Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma. |