Neuropilin 1 Monoclonal antibody
Neuropilin 1 Monoclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig and More (1)
Applications
WB, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2H3F6
Cat no : 60067-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, human plasma, fetal human brain tissue, pig brain tissue |
| Positive IF detected in | SH-SY5Y cells, human embronic stem cells |
| Positive FC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
60067-1-Ig targets Neuropilin 1 in WB, IF, FC, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, Xenopus |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Neuropilin 1 fusion protein Ag0931 |
| Full Name | neuropilin 1 |
| Calculated molecular weight | 103 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC007737 |
| Gene symbol | NRP1 |
| Gene ID (NCBI) | 8829 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neuropilin-1 (NRP1) is a a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Neuropilin 1 antibody 60067-1-Ig | Download protocol |
| IF protocol for Neuropilin 1 antibody 60067-1-Ig | Download protocol |
| FC protocol for Neuropilin 1 antibody 60067-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a. | ||
Am J Med Genet A NRP1 haploinsufficiency predisposes to the development of Tetralogy of Fallot. | ||
Cell Death Dis Cdc42 subcellular relocation in response to VEGF/NRP1 engagement is associated with the poor prognosis of colorectal cancer. | ||
Mol Cell Biochem miR-338 suppresses the growth and metastasis of OSCC cells by targeting NRP1. | ||
Am J Cancer Res CMTM6 promotes cell proliferation and invasion in oral squamous cell carcinoma by interacting with NRP1. | ||
J Pathol Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma. |
