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OXT Polyclonal antibody

OXT Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18041-1-AP

Synonyms

Ocytocin Neurophysin 1, OT, OT NPI, OXT, Oxytocin neurophysin 1, oxytocin, prepropeptide

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pituitary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of human pituitary using 18041-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of human pituitary using 18041-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of mouse brain using 18041-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of mouse ovary using 18041-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of mouse ovary using 18041-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman pituitary tissue, mouse ovary tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18041-1-AP is OXT Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OXT fusion protein Ag12672
Full Name oxytocin, prepropeptide
Calculated Molecular Weight 125 aa, 13 kDa
GenBank Accession NumberBC101841
Gene Symbol OXT
Gene ID (NCBI) 5020
RRIDAB_2918056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for OXT antibody 18041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols