|Positive WB detected in||HepG2 cells, mouse kidney tissue, HUVEC cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||HUVEC cells|
|Positive IHC detected in||human kidney tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Caco-2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 27260-1-AP is Occludin Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse , rat|
|Cited Reactivity||bovine, human, mouse, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Occludin fusion protein Ag26173|
|Calculated molecular weight||522 aa, 59 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC029886|
|Gene ID (NCBI)||4950|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).
|Product Specific Protocols|
|WB protocol for Occludin antibody 27260-1-AP||Download protocol|
|IHC protocol for Occludin antibody 27260-1-AP||Download protocol|
|IF protocol for Occludin antibody 27260-1-AP||Download protocol|
|IP protocol for Occludin antibody 27260-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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