Occludin Polyclonal antibody

Occludin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 27260-1-AP

Synonyms

Occludin, OCLN



Tested Applications

Positive WB detected inmouse liver tissue, HUVEC cells, mouse kidney tissue
Positive IP detected inHUVEC cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27260-1-AP is Occludin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity calf, human, mouse, pig, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Occludin fusion protein Ag26173
Full Name occludin
Calculated molecular weight 522 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC029886
Gene symbol OCLN
Gene ID (NCBI) 4950
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
WB protocol for Occludin antibody 27260-1-APDownload protocol
IHC protocol for Occludin antibody 27260-1-APDownload protocol
IF protocol for Occludin antibody 27260-1-APDownload protocol
IP protocol for Occludin antibody 27260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Carcinog

TIMM50 promotes tumor progression via ERK signaling and predicts poor prognosis of non-small cell lung cancer patients.

Authors - Xiupeng Zhang
humanWB

Sci Rep

Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis.

Authors - Zhaoxing Li
calfWB

J Appl Toxicol

Pharmacological inhibition of TLR4/NF-κB with TLR4-IN-C34 attenuated microcystin-leucine arginine toxicity in bovine Sertoli cells.

Authors - Elikanah Olusayo Adegoke
mouseIF,WB

Clin Sci (Lond)

Activation of G protein-coupled estrogen receptor protects intestine from ischemia/reperfusion injury in mice by protecting the crypt cell proliferation.

Authors - Shiquan Chai
mouseWB

Int J Biol Macromol

Modulating the gut microbiota and inflammation is involved in the effect of Bupleurum polysaccharides against diabetic nephropathy in mice.

Authors - Yuchen Feng
ratWB

Neurochem Res

Ischemia-Reperfusion Injury of Sciatic Nerve in Rats: Protective Role of Combination of Vitamin C with E and Tissue Plasminogen Activator.

Authors - Katerina Apostolopoulou