P27; KIP1 Antibody

P27; KIP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3F12C10

Cat no : 67355-1-Ig

Synonyms

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27KIP1



Tested Applications

Positive WB detected inJurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

67355-1-Ig targets P27; KIP1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P27; KIP1 fusion protein Ag14634
Full Name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Calculated molecular weight 198aa,22 kDa
Observed molecular weight 27 kDa
GenBank accession number BC001971
Gene symbol CDKN1B
Gene ID (NCBI) 1027
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Protocols

Product Specific Protocols
WB protocol for P27; KIP1 antibody 67355-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols