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  • KD/KO Validated

PABPC1,PABP Polyclonal antibody

PABPC1,PABP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10970-1-AP

Synonyms

PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC2, PABPL1, Poly(A) binding protein 1



Tested Applications

Positive WB detected inHCT 116 cells, mouse testis tissue, rat testis tissue, PC-3 cells, HeLa cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman pancreas tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10970-1-AP targets PABPC1,PABP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PABPC1,PABP fusion protein Ag1422
Full Name poly(A) binding protein, cytoplasmic 1
Calculated molecular weight 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC015958
Gene symbol PABPC1
Gene ID (NCBI) 26986
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

Protocols

Product Specific Protocols
WB protocol for PABPC1,PABP antibody 10970-1-APDownload protocol
IHC protocol for PABPC1,PABP antibody 10970-1-APDownload protocol
IF protocol for PABPC1,PABP antibody 10970-1-APDownload protocol
IP protocol for PABPC1,PABP antibody 10970-1-APDownload protocol
FC protocol for PABPC1,PABP antibody 10970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.

Authors - Erika C Urdaneta
humanWB

Cell Rep

Enhancer-Driven lncRNA BDNF-AS Induces Endocrine Resistance and Malignant Progression of Breast Cancer through the RNH1/TRIM21/mTOR Cascade.

Authors - Xiaorong Lin
  • KD Validated
humanWB,IF

Sci Rep

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Authors - S Prpar Mihevc
humanWB

Nat Commun

An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.

Authors - Song Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zee (Verified Customer) (01-28-2020)

It worked very well when I performed western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue
FH

George (Verified Customer) (09-11-2019)

Rb PABP Ig shows very little endogenous background staining with clear dispersed staining seen both in the nucleus and cytoplasm. 90mins heat shock at 42oC led to granular formation in the cytoplasm

  • Applications: Immunohistochemistry,Other,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Spinal Cord/Cortical Slice Culture