- Featured Product
- KD/KO Validated
PABPC1,PABP Polyclonal antibody
PABPC1,PABP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, RIP, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10970-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, mouse testis tissue, PC-3 cells, HeLa cells, A549 cells, rat testis tissue, HT-29 cells, MCF-7 cells, NCI-H1299 cells, HCT-116 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human pancreas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 16 publications below |
| IHC | See 3 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
| RIP | See 1 publications below |
Product Information
10970-1-AP targets PABPC1,PABP in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PABPC1,PABP fusion protein Ag1422 |
| Full Name | poly(A) binding protein, cytoplasmic 1 |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC015958 |
| Gene Symbol | PABPC1 |
| Gene ID (NCBI) | 26986 |
| RRID | AB_10596918 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PABPC1,PABP antibody 10970-1-AP | Download protocol |
| IHC protocol for PABPC1,PABP antibody 10970-1-AP | Download protocol |
| IF protocol for PABPC1,PABP antibody 10970-1-AP | Download protocol |
| IP protocol for PABPC1,PABP antibody 10970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Mol Med The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability | ||
Nat Commun An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis. | ||
J Exp Med E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer. | ||
Clin Transl Med LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing | ||
Leukemia An atlas of bloodstream-accessible bone marrow proteins for site-directed therapy of acute myeloid leukemia. | ||
Haematologica YTHDF3 modulates hematopoietic stem cells by recognizing RNA m6A modification on Ccnd1.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Elisa (Verified Customer) (01-13-2023) | PABPC1 (Poly(A) Binding Protein Cytoplasmic 1) staining (in magenta) and Hoechst (nuclear staining) in blue. PABPC1 ab shows a clear citoplasma staining. Method: RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 10'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).
 |
FH Zee (Verified Customer) (01-28-2020) | It worked very well when I performed western blot.
|
FH George (Verified Customer) (09-11-2019) | Rb PABP Ig shows very little endogenous background staining with clear dispersed staining seen both in the nucleus and cytoplasm. 90mins heat shock at 42oC led to granular formation in the cytoplasm
|
