PAK4/6/7 Polyclonal antibody

PAK4/6/7 Polyclonal Antibody for IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12460-1-AP

Synonyms

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7



Tested Applications

Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12460-1-AP targets PAK4/6/7 in IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAK4/6/7 fusion protein Ag3127
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC024179
Gene symbol PAK7
Gene ID (NCBI) 57144
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. The antibody can recognize PAK4/6/7. PAK4/6/7 belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It acts on a variety of targets.

Protocols

Product Specific Protocols
IHC protocol for PAK4/6/7 antibody 12460-1-APDownload protocol
IP protocol for PAK4/6/7 antibody 12460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols