PAX8 Monoclonal antibody

PAX8 Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C5A11

Cat no : 60144-1-Ig

Synonyms



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

60144-1-Ig targets PAX8 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PAX8 fusion protein Ag0306
Full Name paired box 8
Calculated molecular weight 48 kDa
GenBank accession numberBC001060
Gene symbol PAX8
Gene ID (NCBI) 7849
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356). Proteintech’s 10336-1-AP antibody has cross-reactivities with the other PAX family members.