PCCB Polyclonal antibody

PCCB Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11139-1-AP

Synonyms

PCCase subunit beta, PCCB



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

11139-1-AP targets PCCB in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCCB fusion protein Ag1608
Full Name propionyl Coenzyme A carboxylase, beta polypeptide
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC013768
Gene symbol PCCB
Gene ID (NCBI) 5096
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCCB antibody 11139-1-APDownload protocol
IHC protocol for PCCB antibody 11139-1-APDownload protocol
IF protocol for PCCB antibody 11139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Eur J Immunol

YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

Authors - Satabdi Nandi