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PDE6D Monoclonal antibody

PDE6D Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H4B6

Cat no : 67565-1-Ig

Synonyms

GMP PDE delta, PDE6D, PDED, Protein p17

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of Jurkat using 67565-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis using 67565-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat retina tissue using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of rat retina using 67565-1-Ig

    rat retina tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of mouse brain using 67565-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of human placenta using 67565-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of HeLa using 67565-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of HepG2 using 67565-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, human placenta tissue, rat retina tissue, mouse brain tissue, JAR cells, Pig brain tissue, Rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67565-1-Ig targets PDE6D in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, pig samples.

Tested Reactivity Human, Mouse, Rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDE6D fusion protein Ag29563
Full Name phosphodiesterase 6D, cGMP-specific, rod, delta
Calculated Molecular Weight 17 KDa
Observed Molecular Weight 15-17 kDa
GenBank Accession NumberNM_002601
Gene Symbol PDE6D
Gene ID (NCBI) 5147
RRIDAB_2882779
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.

Protocols

Product Specific Protocols
WB protocol for PDE6D antibody 67565-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Charlotte (Verified Customer) (07-24-2023)

We tested the antibody against PDE6 delta KO cells. We can see the antibody is very noisy but it is working.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: NIH-3T3
PDE6D Antibody Western Blot validation (1/2000 dilution) in NIH-3T3 (Cat no:67565-1-Ig)
FH

Criseyda (Verified Customer) (09-19-2022)

The antibody had background bands/nonspecific bands present but worked best in 5% Milk TBS-T (Blocking, Primary and Secondary) and not 3% BSA TBS-T (Blocking, Primary and Secondary).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T47D
PDE6D Antibody Western Blot validation (1:1000 dilution) in T47D (Cat no:67565-1-Ig)