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  • KD/KO Validated

PFKFB3-Specific Polyclonal antibody

PFKFB3-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13763-1-AP

Synonyms

6PF 2 K/Fru 2,6 P2ase 3, IPFK2, PFK/FBPase 3, PFK2, PFKFB3



Tested Applications

Positive WB detected inmouse spleen tissue, HEK-293 cells, mouse heart tissue, mouse thymus tissue, A431 cells, Jurkat cells
Positive IP detected inHEK-293 cells, mouse spleen tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13763-1-AP targets PFKFB3-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFKFB3-Specific fusion protein Ag4744
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Calculated molecular weight 520 aa, 60 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC040482
Gene symbol PFKFB3
Gene ID (NCBI) 5209
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. It synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5. This antibody is specific to PFKFB3.

Protocols

Product Specific Protocols
WB protocol for PFKFB3-Specific antibody 13763-1-APDownload protocol
IHC protocol for PFKFB3-Specific antibody 13763-1-APDownload protocol
IF protocol for PFKFB3-Specific antibody 13763-1-APDownload protocol
IP protocol for PFKFB3-Specific antibody 13763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Am J Respir Crit Care Med

PFKFB3 in Smooth Muscle Promotes Vascular Remodeling in Pulmonary Arterial Hypertension.

Authors - Laszlo Kovacs
  • KO Validated
mouseIHC,IF,WB

Toxicol Appl Pharmacol

PFKFB3 promotes endotoxemia-induced myocardial dysfunction through inflammatory signaling and apoptotic induction.

Authors - Wen Tian
humanWB

Sci Rep

Andrographolide attenuates imbalance of gastric vascular homeostasis induced by ethanol through glycolysis pathway.

Authors - Huan Yao
humanWB

Biochem Biophys Res Commun

PMID: 30981500

Authors - Qian Long
  • KD Validated
humanWB

J Biol Chem

Increased 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 activity in response to EGFR signaling contributes to non-small cell lung cancer cell survival.

Authors - Nadiia Lypova
humanWB

Cancer Metab

Inhibition of 6-phosphofructo-2-kinase (PFKFB3) induces autophagy as a survival mechanism.

Authors - Alden C Klarer

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hsin-Sheng (Verified Customer) (08-19-2019)

Strong bend intensity at around 60 kD. However, there is a non-specific band near 65-70 kD.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1;200
  • Cell Tissue Type: H1299