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PFKFB3-Specific Polyclonal antibody
PFKFB3-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 13763-1-AP
|Positive WB detected in||mouse spleen tissue, HEK-293 cells, mouse heart tissue, mouse thymus tissue, A431 cells, Jurkat cells|
|Positive IP detected in||HEK-293 cells, mouse spleen tissue|
|Positive IHC detected in||human liver cancer tissue, human kidney tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13763-1-AP targets PFKFB3-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PFKFB3-Specific fusion protein Ag4744|
|Full Name||6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3|
|Calculated molecular weight||520 aa, 60 kDa|
|Observed molecular weight||58 kDa|
|GenBank accession number||BC040482|
|Gene ID (NCBI)||5209|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. It synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5. This antibody is specific to PFKFB3.
|Product Specific Protocols|
|WB protocol for PFKFB3-Specific antibody 13763-1-AP||Download protocol|
|IHC protocol for PFKFB3-Specific antibody 13763-1-AP||Download protocol|
|IF protocol for PFKFB3-Specific antibody 13763-1-AP||Download protocol|
|IP protocol for PFKFB3-Specific antibody 13763-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Am J Respir Crit Care Med
PFKFB3 in Smooth Muscle Promotes Vascular Remodeling in Pulmonary Arterial Hypertension.
Toxicol Appl Pharmacol
PFKFB3 promotes endotoxemia-induced myocardial dysfunction through inflammatory signaling and apoptotic induction.
Andrographolide attenuates imbalance of gastric vascular homeostasis induced by ethanol through glycolysis pathway.
Biochem Biophys Res Commun
J Biol Chem
Increased 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 activity in response to EGFR signaling contributes to non-small cell lung cancer cell survival.
Inhibition of 6-phosphofructo-2-kinase (PFKFB3) induces autophagy as a survival mechanism.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hsin-Sheng (Verified Customer) (08-19-2019)
Strong bend intensity at around 60 kD. However, there is a non-specific band near 65-70 kD.