PHIP Polyclonal antibody
PHIP Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 20933-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, A375 cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | A375 cells |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
20933-1-AP targets PHIP in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PHIP fusion protein Ag14968 |
| Full Name | pleckstrin homology domain interacting protein |
| Calculated Molecular Weight | 1821 aa, 207 kDa |
| Observed Molecular Weight | 207 kDa |
| GenBank Accession Number | BC008909 |
| Gene Symbol | PHIP |
| Gene ID (NCBI) | 55023 |
| RRID | AB_10733522 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHIP antibody 20933-1-AP | Download protocol |
| IF protocol for PHIP antibody 20933-1-AP | Download protocol |
| IP protocol for PHIP antibody 20933-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC Transcription. | ||
Cell Commun Signal RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter | ||
BMC Cancer LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter |
