Tested Applications
| Positive WB detected in | L02 cells, human placenta tissue, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14661-1-AP targets PHLDA2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6331 Product name: Recombinant human PHLDA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-152 aa of BC005034 Sequence: MKSPDEVLREGELEKRSDSLFQLWKKKRGVLTSDRLSLFPASPRARPKELRFHSILKVDCVERTGKYVYFTIVTTDHKEIDFRCAGESCWNAAIALALIDFQNRRALQDFRSRQERTAPAAPAEDAVAAAAAAPSEPSEPSRPSPQPKPRTP Predict reactive species |
| Full Name | pleckstrin homology-like domain, family A, member 2 |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 20-25 kDa |
| GenBank Accession Number | BC005034 |
| Gene Symbol | PHLDA2 |
| Gene ID (NCBI) | 7262 |
| RRID | AB_2163294 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53GA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PHLDA2 antibody 14661-1-AP | Download protocol |
| WB protocol for PHLDA2 antibody 14661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PHLDA2 antibody (Cat# 14661-1-AP) has 9 total citations published in journals including Cell Metabolism, Nature Communications, Aging, Scientific Reports, BMC Cancer, Pathology Research & Practice, Experimental Cell Research, Gene, and Translational Cancer Research. Associated research fields span tumor suppression via ferroptosis, cancer signaling crosstalk, EMT and autophagy in colorectal cancer, lactate metabolism in hepatocellular carcinoma, lung adenocarcinoma immune regulation, drug resistance in colon cancer, and glycolysis in liver cancer.
| Species | Application | Title |
|---|---|---|
Cell Metab PHLDA2-mediated phosphatidic acid peroxidation triggers a distinct ferroptotic response during tumor suppression | ||
Nat Commun Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer. | ||
Aging (Albany NY) PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway.
| ||
BMC Cancer M6A modification-related genes characterize prognostic risk and immune regulation in lung adenocarcinoma. | ||
Pathol Res Pract FOSL1 transcriptionally regulates PHLDA2 to promote 5-FU resistance in colon cancer cells
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