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  • KD/KO Validated

PHLDA2 Polyclonal antibody

PHLDA2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14661-1-AP

Synonyms

BRW1C, BWR1C, HLDA2, IPL, p17 BWR1C, PHLDA2, TSSC3



Tested Applications

Positive WB detected inhuman placenta tissue, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14661-1-AP targets PHLDA2 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity Goat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHLDA2 fusion protein Ag6331
Full Name pleckstrin homology-like domain, family A, member 2
Calculated molecular weight 17 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC005034
Gene symbol PHLDA2
Gene ID (NCBI) 7262
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PHLDA2 antibody 14661-1-APDownload protocol
IHC protocol for PHLDA2 antibody 14661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
GoatWB

Gene

Inhibition of PHLDA2 transcription by DNA methylation and YY1 in goat placenta.

Authors - Ruihong Wang
humanWB

Aging (Albany NY)

PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway.

Authors - Zhan Ma
  • KD Validated