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PI3 Kinase p110 Beta Polyclonal antibody

PI3 Kinase p110 Beta Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IP, IHC, IF, FC, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 20584-1-AP

Synonyms

p110 BETA, PI3 Kinase p110 beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

Validation Data Gallery

  • WB analysis of rat liver using 20584-1-AP

    WB analysis of rat liver using 20584-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 20584-1-AP (PI3 Kinase p110 Beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 20584-1-AP

    IP experiment of HepG2 using 20584-1-AP

    IP Result of anti-PI3K p110(beta) (IP:20584-1-AP, 4ug; Detection:20584-1-AP 1:300) with HepG2 cells lysate 2700ug.

  • IHC staining of human lung cancer using 20584-1-AP

    IHC staining of human lung cancer using 20584-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20584-1-AP (PI3K p110(beta) antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 20584-1-AP

    IHC staining of human lung cancer using 20584-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20584-1-AP (PI3K p110(beta) antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 20584-1-AP

    FC experiment of HeLa using 20584-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PI3 Kinase p110 Beta (20584-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inrat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

20584-1-AP targets PI3 Kinase p110 Beta in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity canine, chicken, fish, human, Megalobrama amblycephala, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phosphoinositide-3-kinase, catalytic, beta polypeptide
Calculated molecular weight 123 kDa
Observed molecular weight 110-130 kDa
GenBank accession numberNM_006219
Gene symbol PIK3CB
Gene ID (NCBI) 5291
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p110 Beta antibody 20584-1-APDownload protocol
IHC protocol for PI3 Kinase p110 Beta antibody 20584-1-APDownload protocol
IP protocol for PI3 Kinase p110 Beta antibody 20584-1-APDownload protocol
FC protocol for PI3 Kinase p110 Beta antibody 20584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
Megalobrama amblycephalaWB

Fish Physiol Biochem

Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala.

Authors - Hualiang Liang
View Article
humanWB

J Biomed Nanotechnol

Nano-Gold Loaded with Resveratrol Enhance the Anti-Hepatoma Effect of Resveratrol In Vitro and In Vivo.

Authors - Duoduo Zhang
View Article
human,mouseWB

Molecules

Secalonic Acid-F, a Novel Mycotoxin, Represses the Progression of Hepatocellular Carcinoma via MARCH1 Regulation of the PI3K/AKT/β-catenin Signaling Pathway.

Authors - Lulu Xie
View Article
ratWB

Int J Mol Med

Fibroblast growth factor-21 alleviates hypoxia/reoxygenation injury in H9c2 cardiomyocytes by promoting autophagic flux.

Authors - Zhong Ren
View Article
humanWB

Inflammation

Umbilical Cord Blood Mesenchymal Stem Cells Enhance Lipopolysaccharide-Induced IL-10 and IL-37 Production in THP-1 Cells.

Authors - Ting Zhou
View Article
humanWB

Life Sci

Dihydroartemisinin attenuates renal fibrosis through regulation of fibroblast proliferation and differentiation.

Authors - Bo Zhang
View Article