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- KD/KO Validated
PI3 Kinase p110 Beta Polyclonal antibody
PI3 Kinase p110 Beta Polyclonal Antibody for IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (5)
Applications
IP, IHC, co-IP, ELISA
Conjugate
Unconjugated
Cat no : 20584-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 91 publications below |
| IHC | See 9 publications below |
Product Information
20584-1-AP targets PI3 Kinase p110 Beta in IP, IHC, co-IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | blunt snout bream, carp, CHICKEN, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | phosphoinositide-3-kinase, catalytic, beta polypeptide |
| Calculated molecular weight | 123 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | NM_006219 |
| Gene symbol | PIK3CB |
| Gene ID (NCBI) | 5291 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PI3 Kinase p110 Beta antibody 20584-1-AP | Download protocol |
| IP protocol for PI3 Kinase p110 Beta antibody 20584-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Fish Physiol Biochem Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala. | ||
J Biomed Nanotechnol Nano-Gold Loaded with Resveratrol Enhance the Anti-Hepatoma Effect of Resveratrol In Vitro and In Vivo. | ||
Molecules Secalonic Acid-F, a Novel Mycotoxin, Represses the Progression of Hepatocellular Carcinoma via MARCH1 Regulation of the PI3K/AKT/β-catenin Signaling Pathway. | ||
Int J Mol Med Fibroblast growth factor-21 alleviates hypoxia/reoxygenation injury in H9c2 cardiomyocytes by promoting autophagic flux. | ||
Inflammation Umbilical Cord Blood Mesenchymal Stem Cells Enhance Lipopolysaccharide-Induced IL-10 and IL-37 Production in THP-1 Cells. | ||
Life Sci Dihydroartemisinin attenuates renal fibrosis through regulation of fibroblast proliferation and differentiation. |
