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PI3K p110(beta) Antibody 36 Publications

Rabbit Polyclonal| Catalog number: 20584-1-AP

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Con: 25 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, co-IP, ELISA
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Images See all

PI3K p110(beta) Antibody WB HepG2 cells 20584-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 20584-1-AP(PI3K p110(beta) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

PI3K p110(beta) Antibody IF HepG2 cells 20584-1-AP

Immunofluorescent analysis of HepG2 cells, using PIK3CB antibody 20584-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

PI3K p110(beta) Antibody IP HepG2 cells 20584-1-AP

IP Result of anti-PI3K p110(beta) (IP:20584-1-AP, 4ug; Detection:20584-1-AP 1:300) with HepG2 cells lysate 2700ug.

PI3K p110(beta) Antibody IHC human lung cancer tissue 20584-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 20584-1-AP( PI3K p110(beta) antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PI3K p110(beta) Antibody IHC human lung cancer tissue 20584-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 20584-1-AP( PI3K p110(beta) antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, chicken, mouse, rat
Positive WB detected in:
HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, rat liver tissue
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human lung cancer tissue, human skin tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:100-1:400
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
phosphoinositide-3-kinase, catalytic, beta polypeptide
Calculated molecular weight:
123 kDa
Observed molecular weight:
110 kDa
GenBank accession number:
NM_006219
Gene ID (NCBI):
5291
Gene symbol
PIK3CB
Synonyms
p110 BETA, PI3 Kinase p110 beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

Background

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.


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