CoraLite®555-conjugated PI3 Kinase p110 Beta Polyclonal antibody

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  IF Staining of HeLa using CL555-20584

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 PI3 Kinase p110 Beta antibody (CL555-20584) at dilution of 1:100.

• Featured Product
• KD/KO Validated

PI3 Kinase p110 Beta Polyclonal Antibody for IF/ICC

Cat No. CL555-20584

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC

Synonyms

PI3K p110(beta), PIK3CB, EC:2.7.1.153, EC:2.7.11.1, p110 BETA

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-20584 targets PI3 Kinase p110 Beta in IF/ICC applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: phosphoinositide-3-kinase, catalytic, beta polypeptide
• Calculated Molecular Weight: 123 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: NM_006219
• Gene Symbol: PI3 Kinase p110 Beta
• Gene ID (NCBI): 5291
• RRID: AB_2919637
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42338
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.