Name: POMC Mouse Monoclonal antibody
Brand: Proteintech
SKU: 66358-1-Ig

Tested Applications

Applications:

WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee

POMC Antibody WB pig brain tissue 66358-1-Ig

pig brain tissue were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

POMC Antibody IHC human pituitary tissue 66358-1-Ig

Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 66358-1-Ig(POMC antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:

human, rat, mouse, pig

Positive WB detected in:

pig brain tissue, rat brain tissue, A375 cells, human placenta tissue, PC-12 cells

Positive IHC detected in:

human pituitary tissue, mouse pancreas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

HeLa cells

Recommended dilution:

WB : 1:1000-1:4000

IHC : 1:500-1:2000

IF : 1:50-1:500

Sample-dependent, check data in Validation tab

Product Information

Source:

Mouse

Purification method:

Protein G purification

Isotype:

IgG1

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

POMC fusion protein Ag17311

Full name:

proopiomelanocortin

Calculated molecular weight:

267aa,29 kDa

Observed molecular weight:

35 kDa

GenBank accession number:

BC065832

Gene ID (NCBI):

5443

Gene symbol

POMC

Synonyms

ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin

Background

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.