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POMC Monoclonal antibody

POMC Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C12C7

Cat no : 66358-1-Ig

Synonyms

ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin

Validation Data Gallery

  • WB analysis of pig brain using 66358-1-Ig

    WB analysis of pig brain using 66358-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66358-1-Ig (POMC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pituitary using 66358-1-Ig

    IHC staining of human pituitary using 66358-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pituitary using 66358-1-Ig

    IHC staining of human pituitary using 66358-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66358-1-Ig

    IF Staining of HeLa using 66358-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66358-1-Ig(POMC antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, rat brain tissue, A375 cells, human placenta tissue, PC-12 cells
Positive IHC detected inhuman pituitary tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66358-1-Ig targets POMC in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen POMC fusion protein Ag17311
Full Name proopiomelanocortin
Calculated molecular weight 267 aa, 29 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC065832
Gene symbol POMC
Gene ID (NCBI) 5443
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.

Protocols

Product Specific Protocols
WB protocol for POMC antibody 66358-1-IgDownload protocol
IHC protocol for POMC antibody 66358-1-IgDownload protocol
IF protocol for POMC antibody 66358-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols