POMC Monoclonal antibody
POMC Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, mouse, pig
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
3C12C7
Cat no : 66358-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig brain tissue, rat brain tissue, A375 cells, human placenta tissue, PC-12 cells |
| Positive IHC detected in | human pituitary tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IF | See 1 publications below |
Product Information
66358-1-Ig targets POMC in WB, IHC, IF,ELISA applications and shows reactivity with human, rat, mouse, pig samples.
| Tested Reactivity | human, rat, mouse, pig |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | POMC fusion protein Ag17311 |
| Full Name | proopiomelanocortin |
| Calculated molecular weight | 267 aa, 29 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC065832 |
| Gene symbol | POMC |
| Gene ID (NCBI) | 5443 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POMC antibody 66358-1-Ig | Download protocol |
| IHC protocol for POMC antibody 66358-1-Ig | Download protocol |
| IF protocol for POMC antibody 66358-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother 919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities. |
