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ACTH Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66358-1-Ig |CloneNo.: 3C12C7

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

ACTH Antibody WB PC-12 cells 66358-1-Ig

PC-12 cells were subjected to SDS PAGE followed by western blot with 66358-1-Ig( POMC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ACTH Antibody IHC human pituitary tissue 66358-1-Ig

Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig( POMC Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ACTH Antibody IHC human pituitary tissue 66358-1-Ig

Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig( POMC Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ACTH Antibody IF HeLa cells 66358-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 66358-1-Ig(POMC antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, rat, mouse
Positive WB detected in:
PC-12 cells, A375 cells, human placenta tissue, rat brain tissue
Positive IHC detected in:
human pituitary tissue, mouse pancreas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACTH fusion protein Ag17311
Full name:
proopiomelanocortin
Calculated molecular weight:
267aa,29 kDa
Observed molecular weight:
34 kDa
GenBank accession number:
BC065832
Gene ID (NCBI):
5443
Gene symbol
POMC
Synonyms
ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, CLIP, CLIP Lipotropin beta, Corticotropin lipotropin, Gamma LPH Melanotropin beta, LPH, MSH, POC, POMC, Pro opiomelanocortin, proopiomelanocortin

Background

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.


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