|Positive WB detected in||mouse heart tissue, rat heart tissue|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11006-1-AP targets PPAN in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PPAN fusion protein Ag1419|
|Full Name||peter pan homolog (Drosophila)|
|Calculated molecular weight||53 kDa|
|Observed molecular weight||55-58 kDa|
|GenBank accession number||BC009833|
|Gene ID (NCBI)||56342|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PPAN (or SSF, SSF1 or SSF2) gene is widely expressed, and encodes peter pan homolog protein which may be localized in nucleus. Two isoforms are produced through alternative splicing. So far the characterization of this protein is largely unknown, multiple phosphorylation sites has been revealed based on high-throughput phosphorylation analysis. This antibody is raised against the C-terminal of huaman PPAN and recognize bands around 55 kDa.
Biochim Biophys Acta Mol Cell Res
Loss of Peter Pan protein is associated with cell cycle defects and apoptotic events.
Loss of Peter Pan (PPAN) Affects Mitochondrial Homeostasis and Autophagic Flux.
Pescadillo homologue 1 and Peter Pan function during Xenopus laevis pronephros development.
J Biol Chem
The Wnt target Peter Pan defines a novel p53-independent nucleolar stress response pathway.
Peter Pan functions independently of its role in ribosome biogenesis during early eye and craniofacial cartilage development in Xenopus laevis.
The long non-coding RNA Paupar promotes KAP1-dependent chromatin changes and regulates olfactory bulb neurogenesis.