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  • KD/KO Validated

PPAN Polyclonal antibody

PPAN Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11006-1-AP

Synonyms

BXDC3, Peter Pan homolog, peter pan homolog (Drosophila), PPAN, SSF, Ssf 1, SSF1, SSF2, Suppressor of SWI4 1 homolog



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11006-1-AP targets PPAN in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPAN fusion protein Ag1419
Full Name peter pan homolog (Drosophila)
Calculated molecular weight 53 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC009833
Gene symbol PPAN
Gene ID (NCBI) 56342
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPAN (or SSF, SSF1 or SSF2) gene is widely expressed, and encodes peter pan homolog protein which may be localized in nucleus. Two isoforms are produced through alternative splicing. So far the characterization of this protein is largely unknown, multiple phosphorylation sites has been revealed based on high-throughput phosphorylation analysis. This antibody is raised against the C-terminal of huaman PPAN and recognize bands around 55 kDa.

Protocols

Product Specific Protocols
WB protocol for PPAN antibody 11006-1-APDownload protocol
IHC protocol for PPAN antibody 11006-1-APDownload protocol
IF protocol for PPAN antibody 11006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Mol Cell Res

Loss of Peter Pan protein is associated with cell cycle defects and apoptotic events.

Authors - Marina Keil
  • KD Validated
humanWB

Cells

Loss of Peter Pan (PPAN) Affects Mitochondrial Homeostasis and Autophagic Flux.

Authors - David P Dannheisig
  • KD Validated
xenopusWB,IHC

Biol Cell

Pescadillo homologue 1 and Peter Pan function during Xenopus laevis pronephros development.

Authors - Tecza Aleksandra A
humanWB, IF

J Biol Chem

The Wnt target Peter Pan defines a novel p53-independent nucleolar stress response pathway.

Authors - Astrid S Pfister
  • KD Validated
humanWB,IF,IP

Development

Peter Pan functions independently of its role in ribosome biogenesis during early eye and craniofacial cartilage development in Xenopus laevis.

Authors - Bugner Verena V
mouseIP

EMBO J

The long non-coding RNA Paupar promotes KAP1-dependent chromatin changes and regulates olfactory bulb neurogenesis.

Authors - Ioanna Pavlaki