|Positive WB detected in||HeLa cells, rat heart tissue|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:600|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
10929-2-AP targets AMPK Alpha 1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AMPK Alpha 1 fusion protein Ag1367|
|Full Name||protein kinase, AMP-activated, alpha 1 catalytic subunit|
|Calculated molecular weight||63 kDa|
|Observed molecular weight||64 kDa|
|GenBank accession number||BC012622|
|Gene ID (NCBI)||5562|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.
Med Sci Monit
The Adenosine Monophosphate (AMP) Analog, 5-Aminoimidazole-4-Carboxamide Ribonucleotide (AICAR) Inhibits Hepatosteatosis and Liver Tumorigenesis in a High-Fat Diet Murine Model Treated with Diethylnitrosamine (DEN).
Baicalin Protects Mice Brain From Apoptosis in Traumatic Brain Injury Model Through Activation of Autophagy.
Eur J Pharmacol
Effect of CAPE-pNO2 against type 2 diabetes mellitus via the AMPK/GLUT4/ GSK3β/PPARα pathway in HFD/STZ-induced diabetic mice.
The NQO1/PKLR axis promotes lymph node metastasis and breast cancer progression by modulating glycolytic reprogramming.
Isodunnianol alleviates doxorubicin-induced myocardial injury by activating protective autophagy.
J Mol Endocrinol
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
LUNFENG (Verified Customer) (01-27-2020)
Jing (Verified Customer) (01-27-2020)
tested in mouse heart lysate, with clear single band around 60 kd.
Jie (Verified Customer) (01-27-2020)
showed a single band at the correct size with mouse heart tissue section
Yan (Verified Customer) (01-03-2020)
We tested this antibody in mouse heart tissue extract. It worked very well and showed a single band around 60kDa.