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PTPN11/SHP2 Polyclonal antibody

Name: PTPN11/SHP2 Polyclonal antibody
Brand: Proteintech
SKU: 20145-1-AP

PTPN11/SHP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20145-1-AP

Synonyms

BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2

Validation Data Gallery

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AllWBIPIHCIF

WB analysis using 20145-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 20145-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 20145-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 20145-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 20145-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 20145-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 20145-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 20145-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 20145-1-AP
IP result of anti-PTPN11/SHP2 (IP:20145-1-AP, 4ug; Detection:20145-1-AP 1:1000) with HEK-293 cells lysate 2800ug.
IHC staining of human breast cancer using 20145-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20145-1-AP (SHP2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 20145-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20145-1-AP (SHP2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 20145-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20145-1-AP (SHP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 20145-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20145-1-AP (SHP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 20145-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 20145-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 20145-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHP2 antibody (20145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 20145-1-AP

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Tested Applications

Positive WB detected in	A431 cells, A549 cells, human brain tissue, human heart tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, U-937 cells, HEK-293 cells, HeLa cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human breast cancer tissue, human gliomas tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)	IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 27 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

20145-1-AP targets PTPN11/SHP2 in WB, IP, IHC, IF, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PTPN11/SHP2 fusion protein Ag13660
Full Name	protein tyrosine phosphatase, non-receptor type 11
Calculated Molecular Weight	597 aa, 68 kDa
Observed Molecular Weight	68-75 kDa
GenBank Accession Number	BC008692
Gene Symbol	PTPN11
Gene ID (NCBI)	5781
RRID	AB_10699877
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PTPN11/SHP2 antibody 20145-1-AP	Download protocol
IHC protocol for PTPN11/SHP2 antibody 20145-1-AP	Download protocol
IF protocol for PTPN11/SHP2 antibody 20145-1-AP	Download protocol
IP protocol for PTPN11/SHP2 antibody 20145-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Hepatology Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients. Authors - Daimin Xiang KD Validated View Article
mouse	WB	Oncogene Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis. Authors - Y Patel View Article
mouse	Cell treatment	Cell Chem Biol Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells. Authors - Rukang Zhang View Article
human	WB	Mol Ther Nucleic Acids miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance. Authors - Tao Han View Article
human	WB	Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. Authors - Hongwei Chu View Article
human	WB	Aging (Albany NY) Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells. Authors - Changying Shi View Article

PTPN11/SHP2 Polyclonal antibody

PTPN11/SHP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 20145-1-AP

WB analysis using 20145-1-AP

WB analysis of NIH/3T3 using 20145-1-AP

WB analysis of U-937 using 20145-1-AP

WB analysis of Jurkat using 20145-1-AP

WB analysis of MCF-7 using 20145-1-AP

WB analysis of human heart using 20145-1-AP

WB analysis of human brain using 20145-1-AP

IP experiment of HEK-293 using 20145-1-AP

IHC staining of human breast cancer using 20145-1-AP

IHC staining of human breast cancer using 20145-1-AP

IHC staining of human gliomas using 20145-1-AP

IHC staining of human gliomas using 20145-1-AP

IHC staining of human heart using 20145-1-AP

IHC staining of human heart using 20145-1-AP

IF Staining of HeLa using 20145-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.

Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.

Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells.

miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance.

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.