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Pericentrin Polyclonal antibody

Pericentrin Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine, mouse

Applications

IF, ELISA

Conjugate

Unconjugated

Cat no : 22271-1-AP

Synonyms

KEN, Kendrin, KIAA0402, MOPD2, PCN, PCNT, PCNT2, PCNTB, PCTN2, pericentrin, Pericentrin B, SCKL4

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Pericentrin Polyclonal antibody (22271-1-AP)

    IF Staining of MDCK using 22271-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 22271-1-AP (Pericentrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Pericentrin Polyclonal antibody (22271-1-AP)

    IF Staining of C2C12 using 22271-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Pericentrin Polyclonal antibody (22271-1-AP)

    IF Staining of HeLa using 22271-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IF detected inMDCK cells, HeLa cells, C2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 3 publications below

Product Information

22271-1-AP targets Pericentrin in IF, ELISA applications and shows reactivity with human, Canine, mouse samples.

Tested Reactivity human, Canine, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name pericentrin
Calculated Molecular Weight 378 kDa
GenBank Accession NumberNM_006031
Gene Symbol PCNT
Gene ID (NCBI) 5116
RRIDAB_2879056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2). The antibody is specific to PCNT, and it can recognize isoform 1 and 2.

Protocols

Product Specific Protocols
IF protocol for Pericentrin antibody 22271-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Elife

De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly.

Authors - Won-Jing Wang
View Article
humanIF

Cell Death Differ

Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis

Authors - Haiyu Song
View Article
humanIF

Cell Rep

Aurora A polyubiquitinates the BRCA1-interacting protein OLA1 to promote centrosome maturation

Authors - Zhenzhou Fang
View Article