Tested Applications
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells, HeLa cells, C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 4 publications below |
Product Information
22271-1-AP targets Pericentrin in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.
| Tested Reactivity | human, mouse, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | pericentrin |
| Calculated Molecular Weight | 378 kDa |
| GenBank Accession Number | NM_006031 |
| Gene Symbol | Pericentrin |
| Gene ID (NCBI) | 5116 |
| RRID | AB_2879056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95613 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2). The antibody is specific to PCNT, and it can recognize isoform 1 and 2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Pericentrin antibody 22271-1-AP | Download protocol |
| IHC protocol for Pericentrin antibody 22271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly. | ||
Cell Death Differ Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis | ||
Cell Rep Aurora A polyubiquitinates the BRCA1-interacting protein OLA1 to promote centrosome maturation | ||
Sci Rep Muscarinic acetylcholine receptor 3 localized to primary endothelial cilia regulates blood pressure and cognition |
