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- KD/KO Validated
QKI Antibody
QKI Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13169-1-AP
Synonyms
HKQ, Hqk, HqkI, Protein quaking, QK, QK1, QK3, QKI
Validation Data Gallery
Tested Applications
| Positive WB detected in | Neuro-2a cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
13169-1-AP targets QKI in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | QKI fusion protein Ag3822 |
| Full Name | quaking homolog, KH domain RNA binding (mouse) |
| Calculated molecular weight | 341aa,38 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC019917 |
| Gene symbol | QKI |
| Gene ID (NCBI) | 9444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
QKI belongs to a family of RNA-binding proteins, which have an HNRNPK homology (KH) domain embedded in a 200-amino acid region called the GSG domain. It involves in regulation of RNA splicing, export of target RNAs from the nucleus, translation of proteins, and RNA stability. Also it required to protect and promote stability of mRNAs such as MBP and CDKN1B. Beside, it acts as a regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for QKI antibody 13169-1-AP | Download protocol |
| IP protocol for QKI antibody 13169-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Exp Pharmacol Physiol LncRNA PTCSC3 inhibits the proliferation, invasion and migration of cervical cancer cells via sponging miR-574-5p. | ||
Naunyn Schmiedebergs Arch Pharmacol Inhibition of miR-574-5p suppresses cell growth and metastasis and enhances chemosensitivity by targeting RNA binding protein QKI in cervical cancer cells. | ||
Front Pharmacol MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI. | ||
J Cell Physiol Quaking promotes the odontoblastic differentiation of human dental pulp stem cells.
| ||
J Biol Chem RNA binding protein Quaking stabilizes Sirt2 mRNA during oligodendroglial differentiation. | ||
J Biol Chem MicroRNA-214 Promotes Dendritic Development by Targeting the Schizophrenia-associated Gene Quaking.
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