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  • KD/KO Validated

QKI Antibody

QKI Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13169-1-AP

Synonyms

HKQ, Hqk, HqkI, Protein quaking, QK, QK1, QK3, QKI



Tested Applications

Positive WB detected inNeuro-2a cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

13169-1-AP targets QKI in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen QKI fusion protein Ag3822
Full Name quaking homolog, KH domain RNA binding (mouse)
Calculated molecular weight 341aa,38 kDa
Observed molecular weight 38 kDa
GenBank accession number BC019917
Gene symbol QKI
Gene ID (NCBI) 9444
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

QKI belongs to a family of RNA-binding proteins, which have an HNRNPK homology (KH) domain embedded in a 200-amino acid region called the GSG domain. It involves in regulation of RNA splicing, export of target RNAs from the nucleus, translation of proteins, and RNA stability. Also it required to protect and promote stability of mRNAs such as MBP and CDKN1B. Beside, it acts as a regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia.

Protocols

Product Specific Protocols
WB protocol for QKI antibody 13169-1-APDownload protocol
IP protocol for QKI antibody 13169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Clin Exp Pharmacol Physiol

LncRNA PTCSC3 inhibits the proliferation, invasion and migration of cervical cancer cells via sponging miR-574-5p.

Authors - Rui Tong
humanWB

Naunyn Schmiedebergs Arch Pharmacol

Inhibition of miR-574-5p suppresses cell growth and metastasis and enhances chemosensitivity by targeting RNA binding protein QKI in cervical cancer cells.

Authors - Rui Tong
humanWB

Front Pharmacol

MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI.

Authors - Xiaosong Wei
human,mouseWB,IHC,IF

J Cell Physiol

Quaking promotes the odontoblastic differentiation of human dental pulp stem cells.

Authors - Shuchen Li
  • KD Validated
humanWB

J Biol Chem

RNA binding protein Quaking stabilizes Sirt2 mRNA during oligodendroglial differentiation.

Authors - Merlin P Thangaraj
humanWB

J Biol Chem

MicroRNA-214 Promotes Dendritic Development by Targeting the Schizophrenia-associated Gene Quaking.

Authors - Koichiro Irie
  • KD Validated