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- KD/KO Validated
RAC1 Monoclonal antibody
RAC1 Monoclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
4A4B11
Cat no : 66122-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, human brain tissue, HeLa cells |
| Positive IP detected in | HL-60 cells, HSC-T6 cells, Raw 264.7 cells, HUVEC cells, HL-60 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | NIH/3T3 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 7 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 66122-1-Ig is RAC1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RAC1 fusion protein Ag17779 |
| Full Name | ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC004247 |
| Gene symbol | RAC1 |
| Gene ID (NCBI) | 5879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAC1 antibody 66122-1-Ig | Download protocol |
| IHC protocol for RAC1 antibody 66122-1-Ig | Download protocol |
| IF protocol for RAC1 antibody 66122-1-Ig | Download protocol |
| IP protocol for RAC1 antibody 66122-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res DRAM2 acts as an oncogene in non-small cell lung cancer and suppresses the expression of p53. | ||
Cell Death Dis MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-β-mediated RAC1 signaling. | ||
Eur J Pharmacol 4-Octyl itaconate protects against renal fibrosis via inhibiting TGF-β/Smad pathway, autophagy and reducing generation of reactive oxygen species. | ||
Acta Pharmacol Sin Asiatic acid prevents renal fibrosis in UUO rats via promoting the production of 15d-PGJ2, an endogenous ligand of PPAR-γ. | ||
Int J Pharm Kidney targeted delivery of asiatic acid using a FITC labeled renal tubular-targeting peptide modified PLGA-PEG system. | ||
Sci Rep The NKD1/Rac1 feedback loop regulates the invasion and migration ability of hepatocarcinoma cells. |
