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RAC1 Monoclonal antibody
RAC1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
4A4B11
Cat no : 66122-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, NIH/3T3 cells, human brain tissue, HeLa cells |
Positive IP detected in | HL-60 cells, HSC-T6 cells, Raw 264.7 cells, HUVEC cells, HL-60 cells, NIH/3T3 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 7 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 66122-1-Ig is RAC1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | RAC1 fusion protein Ag17779 |
Full Name | ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) |
Calculated molecular weight | 21 kDa |
Observed molecular weight | 21 kDa |
GenBank accession number | BC004247 |
Gene symbol | RAC1 |
Gene ID (NCBI) | 5879 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RAC1 antibody 66122-1-Ig | Download protocol |
IHC protocol for RAC1 antibody 66122-1-Ig | Download protocol |
IF protocol for RAC1 antibody 66122-1-Ig | Download protocol |
IP protocol for RAC1 antibody 66122-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Exp Clin Cancer Res DRAM2 acts as an oncogene in non-small cell lung cancer and suppresses the expression of p53. | ||
Cell Death Dis MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-β-mediated RAC1 signaling. | ||
Eur J Pharmacol 4-Octyl itaconate protects against renal fibrosis via inhibiting TGF-β/Smad pathway, autophagy and reducing generation of reactive oxygen species. | ||
Acta Pharmacol Sin Asiatic acid prevents renal fibrosis in UUO rats via promoting the production of 15d-PGJ2, an endogenous ligand of PPAR-γ. | ||
Int J Pharm Kidney targeted delivery of asiatic acid using a FITC labeled renal tubular-targeting peptide modified PLGA-PEG system. | ||
Sci Rep The NKD1/Rac1 feedback loop regulates the invasion and migration ability of hepatocarcinoma cells. |