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Rac1 Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66122-1-Ig |CloneNo.: 4A4B11

Featured Product KD/KO validated

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Con: 105 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Rac1 Antibody WB HSC-T6 cells 66122-1-Ig

HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66122-1-Ig (Rac1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Rac1 Antibody WB NIH/3T3 cells 66122-1-Ig

WB result of RAC1 antibody (66122-1-Ig, 1:2000) with si-control and si-RAC1 transfected NIH3T3 cells.

Rac1 Antibody IHC human colon cancer tissue 66122-1-Ig

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig( RAC1 Antibody) at dilution of 1:400 (under 10x lens).

Rac1 Antibody IHC human colon cancer tissue 66122-1-Ig

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig( RAC1 Antibody) at dilution of 1:400 (under 40x lens).

Rac1 Antibody IF NIH/3T3 cells 66122-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed NIH/3T3 cells using 66122-1-Ig(Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Rac1 Antibody IP HL-60 cells 66122-1-Ig

IP Result of anti-RAC1 (IP:66122-1-Ig, 3ug; Detection:66122-1-Ig 1:700) with HL-60 cells lysate 4000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
HSC-T6 cells, human brain tissue, HL-60 cells, NIH/3T3 cells, HEK-293 cells, RAW 264.7 cells
Positive IP detected in:
HL-60 cells
Positive IHC detected in:
human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
NIH/3T3 cells, HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Rac1 fusion protein Ag17779
Full name:
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Calculated molecular weight:
21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC004247
Gene ID (NCBI):
5879
Gene symbol
RAC1
Synonyms
MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

Background

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.


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