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CoraLite®488-conjugated RAC1 Monoclonal antibody

RAC1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

4A4B11

Cat no : CL488-66122

Synonyms

MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66122-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of CL488-66122 is RAC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAC1 fusion protein Ag17779
Full Name ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC004247
Gene symbol RAC1
Gene ID (NCBI) 5879
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Protocols

Product Specific Protocols
IF protocol for CL488 RAC1 antibody CL488-66122Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Death Dis

MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-β-mediated RAC1 signaling.

Authors - Huiyi Tang