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RAD51 Polyclonal antibody

RAD51 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14961-1-AP

Synonyms

BRCC5, HRAD51, HsRad51, HsT16930, RAD51, RAD51 homolog A, RAD51A, RECA



Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14961-1-AP targets RAD51 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD51 fusion protein Ag6823
Full Name RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC001459
Gene symbol RAD51
Gene ID (NCBI) 5888
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Protocols

Product Specific Protocols
WB protocol for RAD51 antibody 14961-1-APDownload protocol
IHC protocol for RAD51 antibody 14961-1-APDownload protocol
IP protocol for RAD51 antibody 14961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arch Biochem Biophys

MiR-34s negatively regulate homologous recombination through targeting RAD51.

Authors - Shuangjing Chen
humanWB

J Cell Mol Med

RDM1 promotes critical processes in breast cancer tumorigenesis.

Authors - Yajun Chen
humanWB

J Cell Mol Med

Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.

Authors - Xingjie Gao
humanWB

Biomed Pharmacother

Triptolide interferes with XRCC1/PARP1-mediated DNA repair and confers sensitization of triple-negative breast cancer cells to cisplatin.

Authors - Zhiwei Zhang
mouseWB

Cell Cycle

Inhibition of survivin induces spindle disorganization, chromosome misalignment, and DNA damage during mouse embryo development.

Authors - Meng-Hao Pan
humanWB

J Exp Clin Cancer Res

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Authors - Jingyuan Sun