RALB Polyclonal antibody

RALB Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12340-1-AP

Synonyms

RALB, Ras related protein Ral B



Tested Applications

Positive WB detected inY79 cells, human brain tissue, human lung tissue, mouse brain tissue, mouse lung tissue
Positive IHC detected inhuman ovary tumor tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12340-1-AP targets RALB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, Drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RALB fusion protein Ag2992
Full Name v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Calculated Molecular Weight 206 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC018163
Gene Symbol RALB
Gene ID (NCBI) 5899
RRIDAB_2269253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RALB antibody 12340-1-APDownload protocol
IHC protocol for RALB antibody 12340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
DrosophilaIHC

Development

Ral GTPase promotes asymmetric Notch activation in the Drosophila eye in response to Frizzled/PCP signaling by repressing ligand-independent receptor activation.

Authors - Cho Bomsoo B
humanWB

Front Chem

Design, Synthesis, and Biological Evaluation of Pyrano[2,3-c]-pyrazole-Based RalA Inhibitors Against Hepatocellular Carcinoma.

Authors - Yuting Wang
humanWB,IHC

Arch Biochem Biophys

MicroRNA-139 inhibits pancreatic-cancer carcinogenesis by suppressing RalB via the Ral/RAC/PI3K pathway.

Authors - Yan Wang