RANKL Antibody

RANKL Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23408-1-AP

Synonyms

CD254, hRANKL2, ODF, OPGL, OPTB2, Osteoprotegerin ligand, RANKL, sOdf, TNFSF11, TRANCE



Tested Applications

Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 23408-1-AP immunogen is Fusion Protein RANKL protein expressed in E. coli.

Reactivity human
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RANKL fusion protein Ag19975
Full Name tumor necrosis factor (ligand) superfamily, member 11
Calculated molecular weight 317aa,35 kDa
Observed molecular weight
GenBank accession number BC074890
Gene symbol TNFSF11
Gene ID (NCBI) 8600
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.

Protocols

Product Specific Protocols
IHC protocol for RANKL antibody 23408-1-APDownload protocol
IP protocol for RANKL antibody 23408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Med Sci Monit

Fluid Shear Stress Suppresses Osteoclast Differentiation in RAW264.7 Cells through Extracellular Signal-Regulated Kinase 5 (ERK5) Signaling Pathway.

Authors - Chongwen Ma
ratWB

Mol Med Rep

MicroRNA‑21 serves an important role during PAOO‑facilitated orthodontic tooth movement.

Authors - Yuanyuan Zhang
humanIHC

J Histochem Cytochem

Elevated ATF4 Expression in Odontogenic Keratocysts Epithelia: Potential Involvement in Tissue Hypoxia and Stromal M2 Macrophage Infiltration.

Authors - Wen-Qun Zhong
ratIHC

J Periodontal Res

Glyburide inhibits the bone resorption induced by traumatic occlusion in rats.

Authors - Yoichi Arita
humanIHC

Oral Dis

Gingival crevicular fluid levels of SLIT3 are increased in periodontal disease.

Authors - Wei Zhong
ratIHC

Drug Des Devel Ther

Effects Of Triptolide On Tooth Movement And Root Resorption In Rats.

Authors - Fan Yang