RB1 Antibody

RB1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 25628-1-AP

Synonyms

OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1



Tested Applications

Positive WB detected inJurkat cells, A431 cells
Positive IP detected inJurkat cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 25628-1-AP immunogen is Fusion Protein RB1 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RB1 fusion protein Ag22578
Full Name retinoblastoma 1
Calculated molecular weight 928aa,106 kDa
Observed molecular weight 106 kDa
GenBank accession number BC039060
Gene symbol RB1
Gene ID (NCBI) 5925
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RB1 antibody 25628-1-APDownload protocol
IF protocol for RB1 antibody 25628-1-APDownload protocol
IP protocol for RB1 antibody 25628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.

Authors - Yudi Xiong
humanIF

Oncol Lett

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Authors - Kun Lu
humanWB

Oncol Rep

Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma.

Authors - Da-Peng Yun
humanWB

Artif Cells Nanomed Biotechnol

Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway.

Authors - Tianqi Li