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RBPJ Polyclonal antibody

RBPJ Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14613-1-AP

Synonyms

CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, NY-REN-30, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, HeLa cells, HepG2 cells, human heart tissue, human liver tissue, MCF-7 cells, mouse pancreas tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14613-1-AP targets RBPJ in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBPJ fusion protein Ag6206
Full Name recombination signal binding protein for immunoglobulin kappa J region
Calculated molecular weight 56 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC064976
Gene symbol RBPJ
Gene ID (NCBI) 3516
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.

Protocols

Product Specific Protocols
WB protocol for RBPJ antibody 14613-1-APDownload protocol
IHC protocol for RBPJ antibody 14613-1-APDownload protocol
IP protocol for RBPJ antibody 14613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Mycopathologia

M2-Polarized Macrophages Determine Human Cutaneous Lesions in Lacaziosis.

Authors - Tania Cristina Barboza
humanIHC

Med Mycol

Paracoccidioidomycosis: characterization of subpopulations of macrophages and cytokines in human mucosal lesions.

Authors - C Pagliari
humanWB

J Biol Chem

RUNX3 maintains the mesenchymal phenotype after termination of the Notch signal.

Authors - Fu YangXin Y
  • KD Validated