RBX1 Polyclonal antibody

RBX1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14895-1-AP

Synonyms

Protein ZYP, RBX1, Regulator of cullins 1, ring box 1, RING box protein 1, RING finger protein 75, RNF75, ROC1



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14895-1-AP targets RBX1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mitten crabs
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBX1 fusion protein Ag6685
Full Name ring-box 1
Calculated molecular weight 12 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC001466
Gene symbol RBX1
Gene ID (NCBI) 9978
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40.It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.

Protocols

Product Specific Protocols
WB protocol for RBX1 antibody 14895-1-APDownload protocol
IHC protocol for RBX1 antibody 14895-1-APDownload protocol
IP protocol for RBX1 antibody 14895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

Cullin1 binds and promotes NLRP3 ubiquitination to repress systematic inflammasome activation.

Authors - Pin Wan
mitten crabsWB,IF

Gene

Expression pattern and functional analysis of the two RING box protein RBX in spermatogenesis of Chinese mitten crab Eriocheir sinensis.

Authors - Jia-Shun Zhang
humanWB

Cell Death Dis

Bcl-3 regulates TGFβ signaling by stabilizing Smad3 during breast cancer pulmonary metastasis.

Authors - Xi Chen
humanWB

Elife

Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation.

Authors - Lu Lv