|Positive WB detected in||Y79 cells, HeLa cells, mouse eye tissue, PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Sample-dependent, check data in validation data gallery|
17939-1-AP targets RPE65 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RPE65 fusion protein Ag12356|
|Full Name||retinal pigment epithelium-specific protein 65kDa|
|Calculated molecular weight||533 aa, 61 kDa|
|Observed molecular weight||65 kDa, 61 kDa|
|GenBank accession number||BC075036|
|Gene ID (NCBI)||6121|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.
The construction of retinal pigment epithelium (RPE) sheets with enhanced RPE characteristics and cilium assembly using iPS conditioned medium and small incision lenticule extraction derived lenticules.
J Biol Chem
Conversion of all-trans-retinal into all-trans-retinal dimer reflects an alternative metabolic/antidotal pathway of all-trans-retinal in the retina.
Invest Ophthalmol Vis Sci
Retinal Pigment Epithelium Cell Death Is Associated With NLRP3 Inflammasome Activation by All-trans Retinal.
Am J Pathol
Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.