RPE65 Polyclonal antibody

RPE65 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17939-1-AP

Synonyms

LCA2, mRPE65, rd12, Retinoid isomerohydrolase, Retinol isomerase, RP20, RPE65, sRPE65



Tested Applications

Positive WB detected inY79 cells, HeLa cells, mouse eye tissue, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

17939-1-AP targets RPE65 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPE65 fusion protein Ag12356
Full Name retinal pigment epithelium-specific protein 65kDa
Calculated molecular weight 533 aa, 61 kDa
Observed molecular weight 65 kDa, 61 kDa
GenBank accession numberBC075036
Gene symbol RPE65
Gene ID (NCBI) 6121
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.

Protocols

Product Specific Protocols
WB protocol for RPE65 antibody 17939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Biomater

The construction of retinal pigment epithelium (RPE) sheets with enhanced RPE characteristics and cilium assembly using iPS conditioned medium and small incision lenticule extraction derived lenticules.

Authors - Jianing Gu
WB

J Biol Chem

Conversion of all-trans-retinal into all-trans-retinal dimer reflects an alternative metabolic/antidotal pathway of all-trans-retinal in the retina.

Authors - Zhan Gao
human

Invest Ophthalmol Vis Sci

Retinal Pigment Epithelium Cell Death Is Associated With NLRP3 Inflammasome Activation by All-trans Retinal.

Authors - Yi Liao
ratWB

Am J Pathol

Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.

Authors - Volha V Malechka