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S100A12 Polyclonal antibody

S100A12 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC

Conjugate

Unconjugated

Cat no : 16630-1-AP

Synonyms

CAAF1, CAGC, Calgranulin C, CGRP, EN RAGE, ENRAGE, MRP6, Neutrophil S100 protein, p6, Protein S100 A12, S100A12

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A12 Polyclonal antibody (16630-1-AP)

    IHC staining of human tonsillitis using 16630-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A12 Polyclonal antibody (16630-1-AP)

    IHC staining of human tonsillitis using 16630-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A12 Polyclonal antibody (16630-1-AP)

    IHC staining of human tonsillitis using 16630-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A12 Polyclonal antibody (16630-1-AP)

    IHC staining of human tonsillitis using 16630-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A12 Polyclonal antibody (16630-1-AP)

    IHC staining of human colon cancer using 16630-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A12 Polyclonal antibody (16630-1-AP)

    IHC staining of human colon cancer using 16630-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

16630-1-AP targets S100A12 in IHC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A12 fusion protein Ag9961
Full Name S100 calcium binding protein A12
Calculated Molecular Weight 92 aa, 11 kDa
GenBank Accession NumberBC070294
Gene Symbol S100A12
Gene ID (NCBI) 6283
RRIDAB_2878290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A12 is expressed abundantly in granulocytes, and has been implicated to play an important role on inflammatory reactions in various disease states.

Protocols

Product Specific Protocols
IHC protocol for S100A12 antibody 16630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Biol Reprod

Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling.

Authors - Claire Stenhouse
View Article